Peer-reviewed articles 17,970 +



Title: ANTIBACTERIAL POTENTIAL AND CYTOTOXICITY EFFECTS OF SOME PLANTS' CRUDE JUICES

ANTIBACTERIAL POTENTIAL AND CYTOTOXICITY EFFECTS OF SOME PLANTS' CRUDE JUICES
Radwan S. Farag; Layla S. Tawfeek
10.5593/sgem2022V/6.2
1314-2704
English
22
6.2
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
In the latest years, there was an interest in seeking for antimicrobial substances of botanical parts that have capability to suppress foodborne pathogens. Therefore, the antibacterial activity was evaluated on the crude juices produced from the mechanical pressing of some agro and food industrial byproducts, i.e., pomegranate leaves and peels and the leaves of fig, guava and olive. Also, the cytotoxicity effects of these crude juices on normal cells were estimated to check the safety of these crude juices on the human body. The results elucidate that the crude juice of pomegranate peels showed bactericidal ability against all tested bacteria. Olive leaves crude juice exhibited bactericidal ability against S. aureus, B. cereus and E. coli only, while guava leaves crude juice had a bactericidal activity against only S. aureus and P. aeruginosa. Otherwise, pomegranate leaves and fig leaves crude juices did not show any inhibition zones at all. Toxicological potential of crude juices under study revealed no cytotoxic effects at the concentrations used in the current study. Accordingly, pomegranate peels crude juice can be used as a naturalistic antibacterial substance to prohibit the deterioration of stored foods by bacteria moreover, the shelf-life of food would be expanded and massive economic losses due to food wastage would be avoided.
[1] Tako M., Kerekes E.B., Zambrano C., Kotogan A., Papp T., Krisch J., Vagvolgyi C., Plant phenolics and phenolic-enriched extracts as antimicrobial agents against foodcontaminating microorganisms, Antioxidants, vol. 9, pp 1-21, 2020.
[2] Bintsis T., Foodborne pathogens, AIMS Microbiol., vol. 3, pp 529–563, 2017.
[3] Monteiro A.S., Pinto B.L.S., Monteiro J.M., Ferreira R.M., Ribeiro P.C.S., Bando S.Y., Marques S.G., Silva L.C.N., Neto W.R.N., Ferreira G.F., Bomfim M.R.Q., Abreu A.G., Phylogenetic and molecular profile of Staphylococcus aureus isolated from bloodstream infections in Northeast Brazil, Microorganisms, vol. 7, pp 210-213, 2019.
[4] Holzel C.S., Tetens J.L., Schwaiger K., Unraveling the role of vegetables in spreading antimicrobial-resistant bacteria: A need for quantitative risk assessment, Foodborne Pathog. Dis., vol.15/ issue 11, pp 671-688, 2018.
[5] Diggle S.P., Whiteley M., Microbe Profile: Pseudomonas aeruginosa: opportunistic pathogen and lab rat, Microbiol., vol. 166, pp 30–33, 2020.
[6] Erickson M.C., Liao J.Y., Payton A.S., Cook P.W., Ortega Y.R., Survival and internalization of Salmonella and Escherichia coli O157:H7 sprayed onto different cabbage cultivars during cultivation in growth chambers, J. Sci. Food Agric., vol. 99/ issue 7, pp 3530-3537, 2019.
[7] Gutierrez-del-Rio I., Fernandez J., Lombo F., Plant nutraceuticals as antimicrobial agents in food preservation: terpenoids, polyphenols and thiols, Int. J. Antimicrob. Agents, vol. 52, pp 309–315, 2018.
[8] Singh B., Singh J.P., Kaur A., Singh N., Phenolic compounds as beneficial phytochemicals in pomegranate (Punica granatum L.) peel: A review. Food Chem., vol. 261, pp 75–86, 2018.
[9] Pisoschi, A.M.; Pop, A.; Georgescu, C.; Turcu, S.V.; Olah, N.K.; Mathe, E. An overview of natural antimicrobials role in food. Eur. J. Med. Chem. 2018, 143, 922– 935.
[10] Kumar K., Yadav A.N., Kumar V., Vyas P., Dhaliwal H.S., Food waste: a potential bioresource for extraction of nutraceuticals and bioactive compounds, Bioresouz Bioprocess, vol. 4, pp 1-14, 2017.
[11] Capello C., Fischer U. Hungerbuhler K., What is a green solvent? A comprehensive framework for the environmental assessment of solvents, Green Chem., vol. 9, pp 927–934, 2007.
[12] Hindler J.A., Howard B.J., Keiser J.F., Antimicrobial agents and susceptibility testing. In: Howard B.J. (Editor), Clinical and pathogenic microbiology. Mosby year book Inc., St. Louis, MO, USA 1994.
[13] Repetto G., Del Peso A., Zurita J.L., Neutral red uptake assay for the estimation of cell viability/ cytotoxicity, Nature Prot., vol. 3/ issue 7, pp 1125-1131, 2008.
[14] Zambrano C., Kerekes E.B., Kotogan A., Papp T., Vagvolgyi C., Krisch J., Tako M., Antimicrobial activity of grape, apple and pitahaya residue extracts after carbohydrase treatment against food-related bacteria, LWT Food Sci. Technol., vol. 100, pp 416–425, 2019.
[15] Rosas-Burgos E.C., Burgos-Hernandez A., Noguera-Artiaga, L., Kacaniova M., Hernandez-Garcia F., Cardenas-Lopez J.L., Carbonell-Barrachina A.A., Antimicrobial activity of pomegranate peel extracts as affected by cultivar, J. Sci. Food Agric., vol. 97, pp 802–810, 2017.
[16] Farag R.S., Abdel-Latif M.S., Abd El Baky H.H., Tawfeek L.S., Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices, Biotechnol. Rep., vol. 28, pp 1-7, 2020.
[17] Akhtar S., Ismail T., Fraternale D., Sestili P., Pomegranate peel and peel extracts: chemistry and food features, Food Chem., vol. 174, pp 417–425, 2015.
[18] Gorniak I., Bartoszewski R., Kroliczewski J., Comprehensive review of antimicrobial activities of plant flavonoids, Phytochem. Rev., vol. 18, pp 241–272, 2019.
[19] Foss S.R., Nakamura C.V., Ueda-Nakamura T., Cortez D.A., Endo E.H., Dias Filho B.P., Antifungal activity of pomegranate peel extract and isolated compound punicalagin against dermatophytes, Ann. Clin. Microbiol. Antimicrob., vol. 13, pp 32- 38, 2014.
[20] Akbary P., In vitro inhibitory activity of the leaf methanol extract of green tea (Camellia sinensis) against Lactococcus garvieae and Aeromonas hydrophila isolated of rainbow trout (Oncorhynchus mykiss), Adv. Microbiol., vol. 4/ issue 12, pp 829–834, 2014.
[21] Jahromi S.B., Pourshafie M.R., Mirabzadeh E., Tavasoli A., Katiraee F., Mostafavi E., Abbasian S., Punica granatum peel extract toxicity in Mice, Jundishapur J. Nat. Pharm. Prod., vol. 10/ issue 4, pp 1-6, 2015.
[22] Clewell A.E., Beres E., Vertesi A., Glavits R., Hirka G., Endres J.R. Murbach T.S., Szakonyine I.P., A comprehensive toxicological safety assessment of an extract of Olea Europaea L. leaves (Bonolive™), Int. J. Toxicolo., vol. 35/ issue 2, pp 208-221, 2016.
[23] Nirwana I., Rianti D., Soekartono R.H., Listyorini R.D., Basuki D.P., Antibacterial activity of fig leaf (Ficus carica Linn.) extract against Enterococcus faecalis and its cytotoxicity effects on fibroblast cells, Vet World, vol.11/issue 3, pp 342-347, 2018.
Sincere thanks to the Faculty of Agriculture, Cairo University for supporting in fulfillment of this work
conference
Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 06-08 December, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
55-64
06-08 December, 2022
website
8903
Crude juices, Antibacterial activity, MIC, Cytotoxicity

24th SGEM International Conference on Earth & Planetary Sciences


International GeoConference SGEM2024
28 June - 8 July, 2024 / Albena, Bulgaria

Read More
   

SGEM Vienna GREEN "Green Science for Green Life"


Extended Scientific Sessions SGEM Vienna GREEN
25 - 29 November, 2024 / Vienna, Austria

Read More
   

A scientific platform for Art-Inspired Scientists!


The Magical World Where Science meets Art
Vienna, Austria

Read More