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Title: STUDY OF PROPERTIES OF GLUCOSE OXIDASE IMMOBILIZED ON MODIFIED WITH CHITOSAN AND SODIUM TRIPOLYPHOSPHATE MAGNETITE
STUDY OF PROPERTIES OF GLUCOSE OXIDASE IMMOBILIZED ON MODIFIED WITH CHITOSAN AND SODIUM TRIPOLYPHOSPHATE MAGNETITE

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Valentina G. Matveeva; Boris B. Tikhonov; Daniil R. Lisichkin; Ajay Sh. Desai; J.C.S. dos Santos
10.5593/sgem2024/6.1/s25.29
10.5593/sgem2024/6.1
1314-2704
978-619-7603-74-3
English
24
6.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
A new biocatalyst based on glucose oxidase immobilized on Fe3O4 magnetite nanoparticles modified with chitosan and sodium tripolyphosphate was synthesized. Magnetite nanoparticles were obtained by mixing solutions of FeCl2 and FeCl3 with ammonia while heating to a temperature of 65 ?C. To stabilize the nanoparticles and ensure the presence of amino groups on their surface, chitosan and sodium tripolyphosphate were sequentially deposited on magnetite. Immobilization of glucose oxidase on the support was carried out after preliminary activation of the carboxyl groups of the enzyme by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide. The activity and stability of the biocatalyst were investigated in the oxidation reaction of D-glucose to D-glucono-?-lactone. The immobilized biocatalyst was shown to retain more than 60% of the activity compared to the native form of the enzyme. The synthesized biocatalyst can be easily separated from the reaction mixture by a permanent magnet and reused without significant loss of activity. The optimal composition of the biocatalyst providing the maximum activity and operational stability was determined. It has been shown that the immobilization of glucose oxidase on modified magnetite leads to an expansion of the operating range of pH and temperatures by 15-20%, compared with the native enzyme. The successful modification of the magnetite with a fine layer of chitosan as well as the presence of target functional groups on the support surface were confirmed by the Fourier-transform infrared spectroscopy and low-temperature nitrogen adsorption.
[1] Wilson R., Turner A.P.F., Glucose oxidase: an ideal enzyme, Biosensors and Bioelectronics, vol. 7, pp. 165-185, 1992
[2] Bankar S.B., Bule М.V., Singhal R.S., Ananthanarayan L., Glucose oxidase - An overview, Biotechnology Advances, vol. 27, pp. 489–501, 2009.
[3] Tikhonov B.B., Sulman E.M., Stadol'nikova P.Y., Sulman A.M., Golikova E.P., Sidorov A.I., Matveeva V.G., Immobilized enzymes from the class of oxidoreductases in technological processes: A review, Catalisys in Industry, vol.11 (3), pp. 251–263, 2019.
[4] Datta S., Christena L.R., Rajaram Y.R., Enzyme immobilization: an overview on techniques and support materials, 3 Biotechnology, vol. 3(1), pp. 1-9, 2013.
[5] Dong L. C., Wang G., Xiao Y., Xu Y., Zhou X., Jiang H., Luo Q., Immobilization of Glucose Oxidase on a Novel Crosslinked Chitosan Support Grafted with L-Lysine Spacers, Chemical and Biochemical Engineering Quarterly, vol. 25 (3), pp. 395–402, 2011.
[6] Wang X., Zhu K.-X., Zhou H.-M., Immobilization of Glucose Oxidase in Alginate-Chitosan Microcapsules, International Journal of Molecular Sciences, vol. 12, pp. 3042-3054, 2011.
[7] Hashemifard N., Mohsenifar A., Ranjbar B., Allameh A., Lotfi A.S., Etemadikia B., Fabrication and kinetic studies of a novel silver nanoparticles–glucose oxidase bioconjugate, Analytica Chimica Acta, vol. 675, pp. 181–184, 2010.
[8] Wang H., Huang J., Wang C., Li D., Ding L., Han Y. Immobilization of glucose oxidase using CoFe2O4/SiO2 nanoparticles as carrier // Applied Surface Science, vol. 257, pp. 5739–5745, 2011.
[9] Ghadi A., Tabandeh F., Mahjoub S., Mohsenifar A., Roshan F. T., Alavije R., Fabrication and characterization of core-shell magnetic chitosan nanoparticles as a novel carrier for immoblization of Burkholderia cepacia lipase, Journal of Oleo Science, vol. 64(4), pp. 423-430, 2015.
[10] Ein Aliafjeh M., Pourahmad R., Akbari-Adergani B., Azin M., Characteristics of glucose oxidase immobilized on Magnetic Chitosan Nanoparticles, Food Science and Technology, Campinas, vol. 40(1), pp. 68-75, 2019.
[11] Graf E., Penniston J. T., Determination of Hydrogen Peroxide, with its Application Illustrated by Glucose Assay, Clinical Chemistry, vol. 26 (5), pp. 658-660, 1980.
This work was supported by the Ministry of Science and Higher Education, project 075-15-2022-1232.
conference
https://doi.org/10.5593/sgem2024/6.1/s25.29
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Proceedings of 24th International Multidisciplinary Scientific GeoConference SGEM 2024
24th International Multidisciplinary Scientific GeoConference SGEM 2024, 1 - 7 July, 2024
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
191-198
1 - 7 July, 2024
website
9798
magnetite, chitosan, glucose oxidase, immobilization

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