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EFFECTS OF DROUGHT STRESS ON SOME OXIDOREDUCTASE ENZYMES AND PROLINE CONTENT IN LEAVES OF SALIX GENOTYPES
Abstract
The reactive oxygen species are usually generated by normal cellular activities of the aerobic metabolism and play the role of a signal molecule and beneficial growth regulator. When plants are exposed to stress conditions the concentration of reactive oxygen species increases. Therefore, to neutralize these free radicals and to protect cells from oxidative damage enzymatic and non-enzymatic antioxidant systems are activated. In this paper it was investigated the effect of drought on the activities of antioxidant enzymes and on the proline content in leaves of 10 Sallix clones: RO892, RO1077, RO1082, Cozia1, Fragisal, Pesred, Robisal, Inger, Olof and Jorr. The studied plantations were Timburesti area on sandy soil, irrigated and non-irrigated version, comparative to the control plantation Radovan area. The samples were collected in July and there were determined the catalase and peroxidase activity by colorimetric method. The proline content was determined from sulfosalicilc acid extract by colorimetric method with ninhidric acid as reagent using L proline as standard. The obtained results show that the activity of antioxidant enzymes varies with the investigated genotype and with the environmental conditions. In the case of the plants subjected to hydric stress there can be observed an increase in peroxidase activity compared to the irrigated plot. The increase in peroxidase activity suggests a state of oxidative stress, the plants activating a defensive system. The results obtained show an increase in catalase activity and in proline content (with few exceptions) in plants grown on sandy soil in comparison with the control plantation. Measurement of catalase and peroxidase activity and proline content might be used as biomarkers to assess the tolerance of willows for hydric stress
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