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ASPECTS OF GENETIC DIVERSITY OF SILVER LIME (TILIA TOMENTOSA MOENCH.) POPULATIONS IN NATURAL FORESTS OF WESTERN ROMANIA
Abstract
The populations of the native silver lime species (Tilia tomentosa Moench.), one of the most important forest tree species in Western Romania, Caransebes area, show strong vitality and a large morphologic variability. The present carried out research would like to contribute to explaining some aspects of the genetic diversity. The extension of silver lime, during the last decades, in the Caransebes area natural forests (by natural regeneration) seems to be a visible issue in the present-day climate change context. The social values of the silver lime are also important in the researched area making the silver lime a пїЅtree of futureпїЅ both in natural forest and in urban areas. The genetic polymorfism rate was analyzed for twenty provenances of silver lime populations using ISSR (inter simple sequence repeats) primers, considering the advantages of the method. Inter-microsatellite markers are dominant molecular markers, based on PCR (polymerase chain reaction), do not require prior information about the genome and lead to high polymorphism, they are relatively abundant in the genome, highly accurate and display good reproducibility. Ten dominant primers of ISSR were used in this study and the results revealed 232 bands, of which 226 were polymorphic. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis revealed that the groups of silver lime populations have high genetic similarity, supporting the hypothesis that the silver lime provenances belong to that same population which cover a large area in the region.
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References14
Muir N., A survey of the genus Tilia, Plantsman, 5:206-242, 1984;
Svejgaard J., EUFORGEN Technical guidelines for genetic conservation and use for Lime (Tilia spp.), 2003;
Radoglou K., Dobrowolska D., Spyroglou G., Nicolescu V.N., Die Bodenkultur 60(3):7-17, 2009;
Pigott C.D., Francis B., Edinburgh Journal of Botany, United Kingdom 56, 161, 1999;
Tenche-Constantinescu A.M., Modosa E., Chira D., Hernea C., Tenche-Constantinescu R. V., Lalescu D., & Borlea G. F., Tilia spp. Urban trees for future, Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Romania, 43(1), 6, Pp 259-264, 2015;
Haralamb A.T., Cultura speciilor forestiere, Editura Agro-Silvica, Bucuresti, Romania, 1967;
Stanescu, V., Sofletea N., and Popescu O., Flora forestiera lemnoasa a Romaniei, Editura Ceres, Bucuresti, Romania, 1997;
Zietkiewicz E., Rafalski A., Labuda D., Genome fingerprinting by simple sequence repeats (SSR) anchored polymerase chain reaction amplification, Genomics, 20: 176- 183, 1994;
Tsumura Y., Ohba K.and Strauss S.H., Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglasfir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica), Theor Appl Genet 92: 40–45, 1996;
Nagaoka T., Ogihara Y., Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers, Theor. Appl. Genet., 94: 597-602, 1997;
Weir B.S, Genetic data analyisis II, Sinauer Publishers, Sunderland, MA, U.S.A., 1996;
Dangi R.S., Lagu M.D., Choudhary L.B., Ranjekar P.K, Gupta V.S., Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caerulea using ISSR and RAPD markers, BMC Plant Biology, U.S.A, 2004;
Ciulca S., Metodologii de experimentare în agricultură și biologie, Ed Agroprint, Timișoara,, Romania, 2006;
Weising K., DNA fingerprinting in plants: principles, methods and applications, CRC Press,U.S.A., 2005;
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