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In recent years, the input of antibiotics into soils has sharply increased. The environmental behavior of antibiotics is not well known and the precise environmental risk assessment is not practical. Long-term studies have shown the efficiency of the diagnostics and monitoring of soils with biochemical methods, particularly with the help of the indices of soil enzymatic activity. We studied the impact antibiotics (benzylpenicillin, tilosin and nystatin) at different concentrations (100, 500 mg/kg) on hydrolase (ph...