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The assessment of antiseptic solutionsпїЅ efficacy for root canal irrigation in terms of dentinal tubules disinfection is a challenging task. The samples used for this purpose should be standardized according to bacterial counts and the depth of their penetration inside dentinal tubules. However, the samples could be contaminated with bacteria from the surface of dentin during the sample acquisition. The treatment of dentin surface with antiseptic solutions will allow to avoid this contamination and thus to assess...