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Spectrophotometric methods for total protein analysis are generally simple, rapid and sensitive. Such sensitive protein assays may have applications in forensic science, in the detection of protein contaminants in drugs and in a number of other applications of research interest. Biuret reaction with proteins and peptides is widely used in clinical and biological laboratories. In this work, instead of copper sulphate, sodium hydroxide and Seignette salt, we used insoluble copper phosphate, potassium or sodium hydro...

Zein proteins belong to the class of prolamin proteins which, in maize, represent more than 60% of the total endosperm protein. Based on their molecular weight, sequence and solubility, zeins are classified into four different zein structures (?, ?, ?, ?). The major maize seed storage proteins, zeins, are deficient in lysine and tryptophan content, which contribute to the poor nutritional quality of corn. Therefore, we report here on an original and novel process, which consists in successive grinding and sieving ...