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In our study, we optimized two methods of enzyme activity estimations characterizing C and P cycles (?-1,4-glucosidase and acid phosphatase) for agricultural soil with 2.629% of total C and 0.062 % of total N sampled in Tatarstan (Russia). Concentration of the substrates (4-MUF-?-d-glucopyranoside, 4-MUF-phosphate) in the range from 20 to 900 ?mol g-1 soil was used, four levels of buffer pH (3, 6, 8, 11) were compared, and three different dilutions of soil suspension (1%, 2%, 10% w/v) were investigated. Besides, t...

In our study, we optimized two methods of enzyme activity estimations characterizing C and P cycles (?-1,4-glucosidase and acid phosphatase) for agricultural soil with 2.629% of total C and 0.062 % of total N sampled in Tatarstan (Russia). Concentration of the substrates (4-MUF-?-d-glucopyranoside, 4-MUF-phosphate) in the range from 20 to 900 ?mol g-1 soil was used, four levels of buffer pH (3, 6, 8, 11) were compared, and three different dilutions of soil suspension (1%, 2%, 10% w/v) were investigated. Besides, t...