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Ponicka Cave, situated in central Slovakia, is a natural wonder, known for its stunning stalagmites, stalactites, and underground lakes. This unique cave system has a hydrological connection to the Earth's surface, representing a very vulnerable habitat. Long-lasting intensive livestock grazing in the aquifer area of Ponicka cave and insufficiently insulated manure dump were the main reasons for the contamination of this cave. Soil nematodes are the most abundant multicellular organisms on Earth with respect to bo...