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For to determinate the DNA content and analysis of cell distribution in different phases of the cell cycle, flow cytometry was used. The exact measurements of a large number of nuclei by flow cytometry, fluorescently labeled by passing a laser beam (excitation), which marks each individual nucleus by fluorescence, allows a rapid estimation of the relative nuclear content of the DNA of the analyzed plants . Approximately 0.5 cm2 of foliar tissue is harvested, crushed very well, followed by incubation of the sample ...

For to determinate the DNA content and analysis of cell distribution in different phases of the cell cycle, flow cytometry was used. The exact measurements of a large number of nuclei by flow cytometry, fluorescently labeled by passing a laser beam (excitation), which marks each individual nucleus by fluorescence, allows a rapid estimation of the relative nuclear content of the DNA of the analyzed plants . Approximately 0.5 cm2 of foliar tissue is harvested, crushed very well, followed by incubation of the sample ...