Publication
HEAVY METAL BINDING TO PEPTIDES AT HIGHER pH: NOVEL ESI-MS APPROACH
(STEF92 Technology, 2018-06-20, Gabi Drochioiu)
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The stoichiometry of metal?protein complexes is pH dependent. Since mass spectrometric measurements at higher pH values are difficult in the presence of usual buffers, we extended this approach to pH in excess of 10 by adding ethanolamine to the electrospray solution being investigated. Therefore, a new method for probing the metal ion-peptide complexes at high pH utilizing electrospray ionization mass spectrometry (ESI-MS) has been advanced. Nevertheless, the high pH ESI-MS spectra of peptides are far too complex...
Advances in Biotechnology2018