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The oxygen plasma-based bonding of a liver-on-a-chip platform has been optimized for immunostaining and RNA extraction during differentiation of induced pluripotent stem cells into hepatocytes on-chip. The device is a structural and fluidic mimicry of the liverпїЅs lobules, consisting of a double layer of polydimethylsiloxane (PDMS) bonded to glass. This bonding has been adjusted in terms of power, exposure time and post-exposure baking to make the device separable from its glass slide, making cells cultured insid...