Peer-reviewed articles 17,970 +


Title: VALIDATION OF REFERENCE GENES FOR RT-QPCR IN PORCINE OOCYTES CULTURED IN ANTIOXIDANT-ENRICHED MEDIA
VALIDATION OF REFERENCE GENES FOR RT-QPCR IN PORCINE OOCYTES CULTURED IN ANTIOXIDANT-ENRICHED MEDIA

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Oana-Maria Boldura; Jelena Savici; Calin Mircu; Cornel Balta; Simona Marc
10.5593/sgem2024v/4.2/s19.52
10.5593/sgem2024v/4.2
1314-2704
978-619-7603-77-4
English
24
4.2
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Ecology, Biodiversity, and Environmental Protection
Reference genes are essential for normalising data in RT-qPCR experiments and are critical in reducing technical variability. This study aimed to identify and validate stable reference genes for accurate gene expression analysis in porcine oocytes matured in culture media supplemented with antioxidants. Five commonly used reference genes were assessed: PPIA, RPL4, GAPDH, YWHAZ, and TBP, [2, 3] in oocytes cultured under oxidative stress conditions induced by Vitamin C. The experiment involved four experimental groups, analyzed in triplicate using SYBR Green RT-qPCR. PPIA exhibited the lowest SEM, indicating high precision and stability across different experimental conditions. GAPDH showed the most variability, confirming its unsuitability as a reference gene in this context. Differences between groups were statistically significant (p < 0.01) for most genes, with some exceptions for PPIA, YWHAZ, and TBP in specific conditions. The gene stability was evaluated using the NormFinder algorithm, which provided a ranking based on the stability of gene expression across experimental groups. PPIA was found to be the most stable, demonstrating minimal variation in expression levels, while GAPDH was identified as the least stable gene. The findings emphasize the importance of selecting appropriate reference genes for each specific experimental condition to ensure the accuracy of gene expression data.
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https://doi.org/10.5593/sgem2024v/4.2/s19.52
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Proceedings of 24th International Multidisciplinary Scientific GeoConference SGEM 2024
24th International Multidisciplinary Scientific GeoConference SGEM 2024, 27 - 30 November, 2024
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
375-384
27 - 30 November, 2024
website
10140
reference genes, porcine oocytes, gene expression normalization

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