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Title: IN SEARCH OF NEW MOLECULAR MARKERS FOR TAXONOMIC CLASSIFICATION OF CYANOBACTERIA
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IN SEARCH OF NEW MOLECULAR MARKERS FOR TAXONOMIC CLASSIFICATION OF CYANOBACTERIA
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1314-2704
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English
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19
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5.2
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Cyanobacteria are the main components of the phytoplankton in many freshwater and marine ecosystems. So far, about 150 genera and over 2000 species have been described. Although they are one of the most ancient groups of organisms, their taxonomic classification is problematic even today, due to the lack of clear diacritic morphological features between the closely related species, the high variability of the morphological features used for taxonomical purposes depending on the environmental conditions and the occupied ecological niches, as well as the possible horizontal gene transfer.
Consolidated around the idea of applying the principles of the polyphasic taxonomy, the scientists working with cyanobacteria agree that the molecular approach should be the basis for the reorganization of knowledge about Cyanobacteria. It has been accepted that it is better to have more clearly defined monophyletic genera, each of which contains relatively few species than large, poorly defined polyphyletic genera, containing many unrelated species. In this regard, the search, development and application of new molecular-genetic markers for phylogenetic analysis and taxonomic classification of Cyanobacteria is an important task. The larger number of molecular markers would mean a more accurate and precise classification. Therefore, the goal of our study was to develop a new marker that supports and complements the known confirmed markers (16S rDNA, rpoC1, rpoB, gyrB, rbcLX, cpcBA-IGS). Based on the results of the bioinformatics analysis, we think that Psb27 is a suitable candidate for a new molecular marker that can be used for generic and species determination of cyanobacteria. Psb27 is a small (11 kDa) lipoprotein that is part of the photosystem II (PSII) localized in the thylakoid membrane of cyanobacteria. This small protein is suitable since its surface helices III and IV are highly conserved structures responsible for binding to PSII, but the N-terminal part is species-specific. The phylogenetic analyzes performed by various phylogenetic methods and algorithms (minimum evolution, maximum parsimony, maximum-likelihood, and neighbor-joining) based on the Psb27 gene and amino acid sequences, demonstrated its applicability as a molecular marker. The clear separation of the closely related taxonomic categories into monophyletic groups in accordance with the morphological features of the taxa as well as the good correlation with the results from the phylogenetic reconstructions based on 16S rDNA allow us to suggest the Psb27 protein as a new molecular marker that can be used in the taxonomic classification of Cyanobacteria. |
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conference
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19th International Multidisciplinary Scientific GeoConference SGEM 2019
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19th International Multidisciplinary Scientific GeoConference SGEM 2019, 30 June - 6 July, 2019
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Proceedings Paper
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STEF92 Technology
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International Multidisciplinary Scientific GeoConference-SGEM
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Bulgarian Acad Sci; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci & Arts; Slovak Acad Sci; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; World Acad Sci; European Acad Sci, Arts & Letters; Ac
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461-468
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30 June - 6 July, 2019
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website
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cdrom
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6109
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cyanobacteria; photosystem II; Psb27; molecular marker; taxonomy
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