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Title: ZEIN CONJUGATES OF BIOMEDICAL INTEREST: SYNTHESIS AND CHARACTERIZATION BY MALDI-TOF MASS SPECTROMETRY AND FOURIER TRANSFORM INFRARED SPECTROSCOPY
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ZEIN CONJUGATES OF BIOMEDICAL INTEREST: SYNTHESIS AND CHARACTERIZATION BY MALDI-TOF MASS SPECTROMETRY AND FOURIER TRANSFORM INFRARED SPECTROSCOPY
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1314-2704
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English
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21
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6.1
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• Prof. DSc. Oleksandr Trofymchuk, UKRAINE
• Prof. Dr. hab. oec. Baiba Rivza, LATVIA |
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Zein proteins belong to the class of prolamin proteins which, in maize, represent more than 60% of the total endosperm protein. Based on their molecular weight, sequence and solubility, zeins are classified into four different zein structures (?, ?, ?, ?). The major maize seed storage proteins, zeins, are deficient in lysine and tryptophan content, which contribute to the poor nutritional quality of corn. Therefore, we report here on an original and novel process, which consists in successive grinding and sieving of corn seeds to bring about different maize flours, with chemical compositions and protein contents depending on particle hardness. In addition, zeins extracted from maize flours have wide application in: (i) refining food products, such as candies and nuts; (ii) microspheres filled with pesticides are produced out of zein proteins for controlled release of the biologically active substances to provide a safer working environment; (iii) “zein films” are used as a controlled release matrix of active components, such as salicylic acid, lysozyme and EDTA; (iv) coating of tablets and in zein-coated chitosan /tripolyphosphate nanoparticles for oral delivery of selenite. Moreover, the most defining characteristic of zein proteins refers to their insolubility in water and their high solubility in aqueous high percentage alcoholic solutions, such as from 65% to 95% ethanol. Due to the vast genetic polymorphism of the starting material and to the extraction conditions, the consistency of the “zein product” varies substantially; therefore analysis of protein compositions in biological samples of high complexity requires a combination of the latest and modern analytical methods such as SDS-PAGE, 2D gel electrophoresis and mass spectrometry. We have also investigated zein extraction using 65-95% aqueous ethanol under ultrasound conditions from dry-ground whole corn as well as meals with different grain sizes. Besides, the extracted zein and the commercial zein protein were used for different conjugates synthesis. Protein determination was made by UV-Vis spectrophotometry. We have applied modern methods such as MALDI ToF mass spectrometry and FT-IR spectroscopy to characterize the synthesized zein conjugates.
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conference
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21st International Multidisciplinary Scientific GeoConference SGEM 2021
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21st International Multidisciplinary Scientific GeoConference SGEM 2021, 16 - 22 August, 2021
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Proceedings Paper
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STEF92 Technology
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SGEM International Multidisciplinary Scientific GeoConference
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SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci & Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts & Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci
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155-162
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16 - 22 August, 2021
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website
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cdrom
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8282
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zein; mass spectrometry; infrared spectroscopy; conjugates
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