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Title: COMPARISON OF METHODS OF DNA EXTRACTION FROM CRUDE OIL FOR THE FOLLOWING EXPLORING OF ITS BIODIVERSITY
COMPARISON OF METHODS OF DNA EXTRACTION FROM CRUDE OIL FOR THE FOLLOWING EXPLORING OF ITS BIODIVERSITY

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Liliya Biktasheva; Darina Glazunova; Hassan Abdaljalil; Polina Galitskaya
10.5593/sgem2022/1.1/s06.071
10.5593/sgem2022/1.1
1314-2704
978-619-7603-38-5
English
22
1.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Oil and Gas Exploration
The anthropogenic influence on petroleum reservoirs during oil extraction leads to the emergence of new electron acceptors and donors and exogenous microorganisms. Microbial biodegradation of hydrocarbons in petroleum reservoirs leads to a change of its composition, resulting in a decrease in its cost. The identification of microorganisms capable of in-situ degradation of hydrocarbons under reservoir conditions is critical to understand the role of microbes in hydrocarbon conversion. To analyze microbial biodegradation, it is necessary to have information about the composition and structure of microbial communities in petroleum reservoirs. The most complete analysis of the microbial community, the metagenome analysis, can be carried out using the highthroughput sequencing methods. Before sequencing, isolation of DNA should be carried out, and this is a separate task for research due to its difficulty. There are two approaches for DNA extraction - the first is the analysis of the metagenome of the original oils, which allows to deeply explore their biodiversity. The second approach is to conduct pre-cultivation and enrichment of oil microorganisms. The purpose of this study was to compare total DNA extraction methods from oil samples for subsequent analysis of the metagenomes of flooded producing wells. Eighteen crude oil samples were taken from different wells of the Romashkinskoye oil field (Russia) under anaerobic conditions. The direct extraction was successful only for two of them, once using isooctane and once using Winogradsky’s salt solution as eluents. For the other samples, anaerobic enrichment was implemented, is allowed to obtain DNA from 6 more samples. For the last 10 samples, aerobic enrichment was demonstrated to be successful. However, this method of enrichment purely corresponds to the original conditions of the oil well. Further optimization of the direct DNA extraction from crude oil are required.
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This work was supported by the Ministry of Science and Higher Education of the Russian Federation under agreement No. 075-15-2020-931 within the framework of the development program for a world-class Research Center “Efficient development of the global liquid hydrocarbon reserves”.
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https://doi.org/10.5593/sgem2022/1.1/s06.071
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Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 04 - 10 July, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
613-620
04 - 10 July, 2022
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crude oil microbiome, petroleum reservoirs, direct DNA extraction, anaerobic enrichment