Header Image Title: MEDICINAL FUNGI OF THE GENERA OPHIOCORDYCEPS SINENSIS AND PAECILOMYCES HEPIALI
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Title: MEDICINAL FUNGI OF THE GENERA OPHIOCORDYCEPS SINENSIS AND PAECILOMYCES HEPIALI
MEDICINAL FUNGI OF THE GENERA OPHIOCORDYCEPS SINENSIS AND PAECILOMYCES HEPIALI
Anna Uhrinova; Lucia Ungvarska Malucka; Martin Pavlik
10.5593/sgem2022/6.1/s25.12
10.5593/sgem2022/6.1
1314-2704
978-619-7603-48-4
English
22
6.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
Various secondary metabolites isolated from Ophiocordyceps sinensis, the Chinese caterpillar fungus, have been reported to possess high therapeutic potential. Paecilomyces hepiali has even more biologically important chemical components with interesting pharmacological activity. Traditional Chinese medicine uses Ophiocordyceps sinensis to treat lung and respiratory system problems, hyposexuality, hypolipidemia kidney, or liver, heart, and immune system conditions. In addition, it has been used to treat various forms of cancer, especially in addition to chemotherapy and radiotherapy. The objective of the present study was to determine the antioxidant activity of metabolites isolated from medicinal fungi of the genera Ophiocordyceps sinensis and Paecilomyces hepiali cultivated on two types of rice (Oryza sativa var. indica and Oryza sativa var. japonica). Antioxidant activity was determined by applying the 1,1-diphenyl-2-picrylhydrazyl radical scavenging method to alcoholic extracts of O. sinensis and P. hepiali strains. Samples were prepared using reflux extractions. Refluxing produced the highest extraction yield. The highest extraction yield was achieved for the O. sinensis (4OS, 9.86 %) sample grown on Oryza sativa var. japonica. The highest scavenging ability for the stable 1,1-diphenyl-2-picrylhydrazyl radical was observed for the extract of Paecilomyces hepiali cultivated on Oryza sativa var. japonica (Sample 5PH: IC50DPPH 3.03 mg/mL). The chemical structure of the alcohol extracts was determined by NMR spectroscopy. Unsaturated fatty acids (Z-oleic acid, linoleic acid and D-mannitol) were identified as the major components of the extracts.
Ophiocordyceps sinensis, Paecilomyces hepiali, Oryza sativa var. indica, Oryza sativa var. japonica, reflux extraction
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For measuring infrared spectra, we thank Martin Vavra, PhD. from the University of Pavol Jozef Safarik in Kosice, Department of Inorganic Chemistry. For measuring NMR spectra we thank Maria Vilkova, PhD. and associate professor Jan Imrich, PhD. from the University of Pavol Jozef Safarik in Kosice, Nuclear Magnetic Spectroscopy Laboratory. For providing samples of fungi, we thank Martin Rajtar (Mykoforest Velcice, Slovakia). The research results are presented within the projects of the KEGA Grant Agency (no. 002TU Z-4/2022) and the VEGA Grant Agency (no. 1/0071/21).
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https://doi.org/10.5593/sgem2022/6.1/s25.12
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Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 04 - 10 July, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
97-104
04 - 10 July, 2022
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