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Title: SYNTHESIS OF MAGNETICALLY SEPARATED BIOCATALYTIC SYSTEMS
SYNTHESIS OF MAGNETICALLY SEPARATED BIOCATALYTIC SYSTEMS

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Olga Grebennikova; Aloeksandrina Sulman; Valentina Matveeva
10.5593/sgem2022/6.1/s25.16
10.5593/sgem2022/6.1
1314-2704
978-619-7603-48-4
English
22
6.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
The use of magnetic nanoparticles in biocatalysis, due to their unique properties, such as controlled particle size, large surface area, and ease of separating them and the reaction mixture by applying an external magnetic field, makes it possible to reuse enzymes immobilized on magnetic nanoparticles for catalytic processes. In this work, horseradish root peroxidase was immobilized on Fe3O4 magnetic nanoparticles. The carrier surface was modified and activated before enzyme immobilization using 3- aminopropyltriethoxysilane and glutaraldehyde. Testing of biocatalytic systems was carried out in the oxidation reaction of 2,2'-azino-bis-(3-ethylbenzthiozolin-6-sulfonic acid) diammonium salt with hydrogen peroxide. The immobilized enzyme showed high efficiency and stability compared to the native enzyme. Also, in the work, the joint immobilization of peroxidase and glucose oxidase on magnetically attached carriers was studied. Enzymes were immobilized on Fe3O4 magnetic nanoparticles and SiO2. Optimal conditions (temperature, pH) were selected for all biocatalytic systems.
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The current work was financially supported by the Russian Science Foundation (grant 22-24-20027).
conference
https://doi.org/10.5593/sgem2022/6.1/s25.16
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Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 04 - 10 July, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
129-136
04 - 10 July, 2022
website
8644
biocatalysis, immobilization, peroxidase, glucose oxidase, magnetic nanoparticles