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Title: BIOCHAR ENHANCES ANTIBIOTIC-RESISTANT GENES REMOVAL FROM AQUEOUS ECOSYSTEMS
BIOCHAR ENHANCES ANTIBIOTIC-RESISTANT GENES REMOVAL FROM AQUEOUS ECOSYSTEMS

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Natalia Danilova; Kamalya Karamova; Polina Galitskaya
10.5593/sgem2022/5.1/s20.008
10.5593/sgem2022/5.1
1314-2704
978-619-7603-46-0
English
22
5.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Ecology and Environmental Protection
Pollution of aquatic ecosystems with antibiotic-resistant genes originating from human medicine and veterinary is an urgent problem due to the potential threaten to public health. Antibiotic-resistant genes enter surface waters and wastewater through vertical and horizontal water runoff. At the same time, heavy metals and biogenic substances often presented in aqueous ecosystems often exacerbate the problem since the drive the horizontal transfer of antibiotic-resistance genes. To solve the problem of purification of waters from antibiotic-resistant genes, the adsorbing agents, such as biochar, might be used. In this work, we studied the effect of biochar on the dynamics of the content of tetracycline-resistant genes in a liquid LB medium with a microbial community transferred to the medium from compost. The following additives were used - Cu (600 µg*l-1), Cd (130 µg*l-1), Ni (70 µg*l-1), Fe (1500 µg*l-1), humic acids (6%), oxytetracycline (300 mg/l). Real-time PCR revealed the absence of the tet(O) gene both in all variants with and without biochar. The highest excesses over control were found for the tet(M) and tet(C) genes. The introduction of biochar made it possible to reduce the content of antibiotic-resistant genes in all samples with different additives. Thus, in the variant with Cd, the content of the tet(A), tet(B), tet(C) и tet(S) gene was eliminated. The tet(А), tet(E) и tet(S) genes were completely absent in the sample with antibiotic.
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The work is carried out in accordance with the Strategic Academic Leadership Program "Priority 2030" of the Kazan Federal University of the Government of the Russian Federation.
conference
https://doi.org/10.5593/sgem2022/5.1/s20.008
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Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 04 - 10 July, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
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04 - 10 July, 2022
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antibiotic resistance, antibiotic-resistant genes, biochar, aqueous ecosystems, wastewater