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Title: CHALLENGES ASSOCIATED WITH BARCODING METHOD
CHALLENGES ASSOCIATED WITH BARCODING METHOD

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Dana Szaboova; Eliska Gburova Stubnova; Ivona Kautmanova
10.5593/sgem2022/5.1/s20.012
10.5593/sgem2022/5.1
1314-2704
978-619-7603-46-0
English
22
5.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Ecology and Environmental Protection
The DNA barcoding project provides a very quick and easy way to identify different types of organisms based on their DNA. The main objective of this study is to obtain the sequences of different species of fungi, plants and animals to map the biodiversity of Slovakia. This project also represents a new use for collections stored in natural history museums around the world. This paper shows in particular a laboratory view of the DNA barcoding project, such as the practical application of the methodology for different groups of organisms, the possibilities of using different genes and emphasizes the importance of professional taxonomists. In the museum, we deal with a wide range of samples and we encounter various difficulties. For example, some genera of fungi contain unreadable sections in the middle of the sequence, in insect groups it is a problem with samples killed with ethyl acetate or too old samples, where the DNA is mostly degraded. For the amplification of bird samples, we have found no consistent method for the whole group and we work with several different primer sets and conditions to be able to amplify most of the samples. The identification of various plant species according to their barcodes has also proven quite difficult, as plants are a very specific and fast-evolving group of organisms, and their distinction according to the short barcode regions is nearly impossible. Therefore, we would like to emphasize the necessity of cooperation with specialized taxonomists. Our data are continuously uploaded to the international BOLD database, where there are already more than 500 different species of a wide range of groups of fungi, plants and animals from Slovakia.
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Research was funded by Operational Program of Integrated Infrastructure: “DNA barcoding of Slovakia (SK-BOL), as a part of international initiative International Barcode of Life (iBOL)” (ITMS2014+313021W683).
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https://doi.org/10.5593/sgem2022/5.1/s20.012
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Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 04 - 10 July, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
83-90
04 - 10 July, 2022
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DNA barcoding, Slovak biodiversity, difficulties, methods