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Title: NEW MICROBIAL BIOPREPARATION FOR AGRICULTURE CONSISTING OF CONSORTIUM OF BIOSURFACTANT PRODUCERS
NEW MICROBIAL BIOPREPARATION FOR AGRICULTURE CONSISTING OF CONSORTIUM OF BIOSURFACTANT PRODUCERS

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Gulnaz Galieva; Natalia Danilova; Svetlana Selivanovskaya; Polina Galitskaya
10.5593/sgem2022/5.1/s20.041
10.5593/sgem2022/5.1
1314-2704
978-619-7603-46-0
English
22
5.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Ecology and Environmental Protection
The present work is an attempt to create a biosurfactant producing consortia on the bases of initial rhizospheric community of lettuce plant (Lactuca sativa). To obtain consortia, 47 strains from the rhizosphere were isolated and checked upon their ability to produce biosurfactants. The ability of the isolates to produce biosurfactants was analyzed on the basis of their ability to emulsify crude oil (E24 index). The isolates with the highest E24 were Bacillus. oryzaecorticis (80%), B. simplex (65%), Paenibacillus xylanilyticus (60%), and P. illinoisensis (73%). These isolates (numbered further as 1, 2, 3 and 4, respectively) were further cultivated together in consortia of two, three or four members. The cultivation was conducted of LB and BH medium during 72 h, after that the abundance of the consortium members as well as the ability of the consortium to utilize different carbon substrates (Biolog EcoPlate® system) were assessed.
It was found out, that isolate 1 was not able to grow in consortia. Other isolates were able to grow in combinations with each other, at least in one of the media. Isolate 4 survived in all the combinations investigated. Among consortia investigated, the two-members consortium 3-4 was able to survive in both media.
The AWCD index reflects the average ability of the microbes to utilize 31 different carbon substrates. For individual isolates 1, 2, 3 and 4, AWCD was estimated to be 0.07, 0.04, 0.19 and 0.25, respectively. It exceeded the initial levels of AWCD demonstrated by the individual isolates only in 3 cases: 1-3-4 (0.26), 1-2-3-4 (0.32), 2-4 (0.59). It can be concluded that consortia are able to survive in larger spectrum of environmental niches as compared with individual isolates, however, competition between the consortium members limits their active growth.
[1] Martin-Guay M. O., Paquette A., Dupras J., Rivest D., The new Green Revolution: Sustainable intensification of agriculture by intercropping,Science of The Total Environment, 2018, vol. 615, pp. 767–772.
[2] Raj A., Kumar A., Dames J. F., Tapping the Role of Microbial Biosurfactants in Pesticide Remediation: An Eco-Friendly Approach for Environmental Sustainability,Frontiers in Microbiology, 2021, vol. 12, pp. 3886.
[3] Sachdev D. P., Cameotra S. S., Biosurfactants in agriculture,Applied Microbiology and Biotechnology, 2013, vol. 97, pp. 1005.
[4] Gayathiri E., et al., Biosurfactants: Potential and Eco-Friendly Material for Sustainable Agriculture and Environmental Safety—A Review,Agronomy 2022, Vol. 12, Page 662, 2022, vol. 12, pp. 662.
[5] Crouzet J., et al., Biosurfactants in Plant Protection Against Diseases: Rhamnolipids and Lipopeptides Case Study,Frontiers in Bioengineering and Biotechnology, 2020, vol. 8, pp. 1014.
[6] Fatima S., et al., Bioremediation of contaminated soils by bacterial biosurfactants,Advances in Remediation Techniques for Polluted Soils and Groundwater, 2022, pp. 67–85.
[7] Abdel-Mawgoud A. M., Lepine F., Deziel E., Rhamnolipids: diversity of structures, microbial origins and roles,Applied microbiology and biotechnology, 2010, vol. 86, pp. 1323–1336.
[8] Gao L., et al., Plipastatin and surfactin coproduction by Bacillus subtilis pB2-L and their effects on microorganisms,Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 2017, vol. 110, pp. 1007–1018.
[9] Elumalai P., et al., Enhanced biodegradation of hydrophobic organic pollutants by the bacterial consortium: Impact of enzymes and biosurfactants,Environmental Pollution, 2021, vol. 289, pp. 117956.
[10] Rutgers M., et al., Monitoring soil bacteria with community-level physiological profiles using BiologTM ECO-plates in the Netherlands and Europe,Applied Soil Ecology, 2016, vol. 97, pp. 23–35.
[11] Sun W., et al., Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp. CQ2 from Changqing oil field, China,RSC Advances, 2018, vol. 8, pp. 39710–39720.
[12] Kumar S., Stecher G., Li M., Knyaz C., Tamura K., MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms,Molecular biology and evolution, 2018, vol. 35, pp. 1547–1549.
[13] Galieva G. S., Gilmutdinova I. M., Fomin V. P., Selivanovskaya S. Y., Galitskaya P. Y., Monitoring Soil Bacteria with Community-Level Physiological Profiles Using BiologTM ECO-Plates in the Republic of Tatarstan (Russia),IOP Conference Series: Earth and Environmental Science, 2018, vol. 107.
[14] Al-Dhabaan F. A. M., Bakhali A. H., Analysis of the bacterial strains using Biolog plates in the contaminated soil from Riyadh community,Saudi Journal of Biological Sciences, 2017, vol. 24, pp. 901–906.
[15] Beisel J. N., Moreteau J. C., A simple formula for calculating the lower limit of Shannon’s diversity index,Ecological Modelling, 1997, vol. 99, pp. 289–292.
This work was funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities, project № 0671-2020-0055.
conference
https://doi.org/10.5593/sgem2022/5.1/s20.041
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Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 04 - 10 July, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
319-326
04 - 10 July, 2022
website
8705
Plant-associated microbiome, biosurfactant, growth rate, Biolog EcoPlate®, bacterial consortium