Peer-reviewed articles 17,970 +


Jitka Mala; Kristina Panikova; Zuzana Bilkova
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
The use of pesticides has negative effects on the quality of groundwater. The triazine pesticide atrazine has not been applied to soil in agriculturally used areas of the Czech Republic for many years, yet atrazine and its metabolites are detected in groundwater throughout the country. The effect of these substances on the denitrification process that takes place in groundwater is unclear.
This study aims to examine the behavior of atrazine under denitrifying conditions and the pesticide’s effect on the denitrification process. 7-day and 28-day laboratory tests at a concentration of 100 ?g/L atrazine were performed to simulate such conditions. A single dose of atrazine was introduced to the samples at the beginning of the tests.
No inhibition of the denitrification process was detected in any of the tests. Stimulation of denitrification was measured in the last week of the 28-day test. Adsorption on poplar shavings was the dominant process of the decrease in atrazine concentration during both the 7- and 28-day tests. The biotic loss was 3.9% for the 7-day test. In the 28-day test, there was a total biotic loss of 12.1%. A similar biotic loss of 9.8% was measured also in a 7-day test performed at higher pH. At the end of the 28-day test, the transformation product atrazine-2-hydroxy was detected in the supernatant in very low concentrations. A significant effect of the HgCl2 inhibitor on the instantaneous adsorption rate was observed for all tests.
[1] Kodes, V., Problematika pesticidu v ochrane vod – jaka data mame k dispozici a co nam rikaji?, Bienalni konference Voda, Czech Republic, 2019, pp 18-25.
[2] Jablonowski, N. D., Schaffer, A., & Burauel, P., Still present after all these years: persistence plus potential toxicity raise questions about the use of atrazine, Environmental Science and Pollution Research, vol. 18/issue 2, pp 328-331, 2011.
[3] Navarro, S., Vela, N., Jose Gimenez, M., & Navarro, G., Persistence of four striazine herbicides in river, sea and groundwater samples exposed to sunlight and darkness under laboratory conditions, Science of The Total Environment, vol. 329/issue 1-3, pp 87-97, 2004.
[4] Douglass, J. F., Radosevich, M., & Tuovinen, O. H., Biomineralization of atrazine and analysis of 16S rRNA and catabolic genes of atrazine degraders in a former pesticide mixing site and a machinery washing area, Journal of Soils and Sediments, vol. 16/issue 9, pp 2263-2274, 2016.
[5] Ilhan, Z. E., Ong, S. K., & Moorman, T. B., Dissipation of Atrazine, Enrofloxacin, and Sulfamethazine in Wood Chip Bioreactors and Impact on Denitrification, Journal of Environmental Quality, vol. 40/issue 6, pp 1816-1823, 2011.
[6] Panikova, K., Optimalizace metodiky pro testovani pesticide, - vodarensky informacni portal [online], 2021.
[7] Zhao, J., He, Q., Chen, N., Peng, T., & Feng, C., Denitrification behavior in a woodchip-packed bioreactor with gradient filling for nitrate-contaminated water treatment, Biochemical Engineering Journal, vol. 154, 2020.
[8] Hu, R., Zheng, X., Zheng, T., Xin, J., Wang, H., & Sun, Q., Effects of carbon availability in a woody carbon source on its nitrate removal behavior in solid-phase denitrification, Journal of Environmental Management, vol. 246, pp 832-839, 2019.
[9] Qian, W., Ma, B., Li, X., Zhang, Q., & Peng, Y., Long-term effect of pH on denitrification: High pH benefits achieving partial-denitrification, Bioresource Technology, vol. 278, pp 444-449, 2019.
[10] Daigger, G. T., Adams, C. D., & Steller, H. K., Diffusion of oxygen through activated sludge flocs: experimental measurement, modeling, and implications for simultaneous nitrification and denitrification, Water Environment Research, vol. 79/issue 4, pp 375-387, 2007.
[11] Lahdhiri, A., Lesage, G., Hannachi, A., & Heran, M., Minimum COD needs for denitrification: from biological models to experimental set-up. Desalination and Water Treatment, vol. 61, pp 326-334, 2017.
[12] Krause Camilo, B., Bioreactor reduces atrazine and nitrate in tile drain waters, Ecological Engineering, vol. 86, pp 269-278, 2016.
[13] Cesar, A., & Ros, M., Long-term study of nitrate, nitrite and pesticide removal from groundwater: A two-stage biological process, International Biodeterioration & Biodegradation, vol. 82, pp 117-123, 2013.
[14] Yue, L., Ge, C. J., Feng, D., Yu, H., Deng, H., & Fu, B., Adsorption–desorption behavior of atrazine on agricultural soils in China, Journal of Environmental Sciences, vol. 57, pp 180-189, 2017.
[15] Douglass, J. F., Radosevich, M., & Tuovinen, O. H., Mineralization of atrazine in the river water intake and sediments of a constructed flow-through wetland, Ecological Engineering, vol. 72, pp 35-39, 2014.
This research was financially supported by the Brno University of Technology Junior Specific Research Project FAST-J-22-7911 - Denitrification and degradation of pesticides. The authors thank the RECETOX institute and ALS Czech Republic
Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 06-08 December, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
06-08 December, 2022
denitrification, atrazine, inhibition, wood shavings