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Title: AEROBIC BACTERIA FROM THE OILWELLS OF THE SUPERGIANT ROMASHKINSKOYE OILFIELD
AEROBIC BACTERIA FROM THE OILWELLS OF THE SUPERGIANT ROMASHKINSKOYE OILFIELD

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Liliya Biktasheva; Natalia Danilova; Polina Galitskaya; Svetlana Selivanovskaya; Maria Shipaeva
10.5593/sgem2022V/6.2/s25.07
10.5593/sgem2022V/6.2
1314-2704
978-619-7603-52-1
English
22
6.2
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
Many oilfields such as supergiant Romaschkinskoye oilfield in Russia contain heavy oils that are complicated to recover. In order to increase the proportion of obtained oil, methods of enhanced oil recovery are implemented, and microbial methods (MEOR) are considered as safe and efficient once. In-situ MEOR are based on stimulation of oilwell microflora that partly decomposes hydrocarbon molecules or alters oil-water interface tension. Despite oilwells are an environments with anaerobic conditions, many allochtonous microbes there are able to use aerobic pathways. Moreover, stimulation of those aerobic microbes by addition of nutrients and oxygen (that is contained in the production water) results in significant oil recovery increase since aerobes produce metabolites that activate the microflora of the lower anaerobic zone. However, aerobic microbial communities of many oilwells remain unstudied and their role for oil recovery is underestimated.
The purpose of the present study was to reveal the biodiversity and oil-emulsifying ability of the aerobic microorganisms inhabiting heavy oils from the supergiant Romaschkinskoye oilfield. For this purpose, oil was sampled from 5 oil reservoirs belonging to the Romaschkinskoye oilfield. For each reservoir, oil was obtained from 3 different wells recovering oil from different depths. After cultivation under aerobic conditions, 16 isolates belonging to Bacillus and Enterococcus genera were obtained from the samples. The emulsification index (E24) revealed for cultural media of those isolates ranged between 15 and 70%. For six isolates, it exceeded 60% which can be promising for using MEOR. Further investigation of the ability of those isolates to stimulate the oil anaerobic microflora is required.
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This work was supported by the Ministry of Science and Higher Education of the Russian Federation under agreement No. 075-15-2022-299 within the framework of the development program for a world-class Research Center “Efficient development of the global liquid hydrocarbon reserves”.
conference
https://doi.org/10.5593/sgem2022V/6.2/s25.07
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Proceedings of 22nd International Multidisciplinary Scientific GeoConference SGEM 2022
22nd International Multidisciplinary Scientific GeoConference SGEM 2022, 06-08 December, 2022
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
47-54
06-08 December, 2022
website
8902
MEOR, aerobic bacteria, biosurfactant, oilwells