Peer-reviewed articles 17,970 +



Title: TOTAL PHENOLIC CONTENT, FLAVONOID CONTENT AND ANTIOXIDANT POTENTIAL OF BILBERRY (Vaccinium myrtillus L.) FROM U.P. V HIGIGEL

TOTAL PHENOLIC CONTENT, FLAVONOID CONTENT AND ANTIOXIDANT POTENTIAL OF BILBERRY (Vaccinium myrtillus L.) FROM U.P. V HIGIGEL
Petru Ioan Dragomir; Beatrice Otilia Saiti; Camelia Tulcan; Maria Mihaela Moatar; Dorin Dumitru Camen
10.5593/sgem2023/3.1
1314-2704
English
23
3.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Bilberries selected from U.P. V. Higigel were investigated for their antioxidative potential using 2,2-dyphenyl-1-picrylhydrazyl (DPPH) scavenging. In order to determine the total content of polyphenols, the method described by Tamas-Krumpe Octavia and collaborators in 2010 [1] was chosen, consisting in the use of Folin-Ciocalteu reagent 0.2 N and sodium carbonate (Na2CO3) and the use of gallic acid in different concentrations as a standard. The spectrophotometric determination method based on the formation of the aluminum complex is the most widely used method for the analysis of flavonoid content in plant matrices.
The extract of Vaccinium myrtillus L. in ethanol, obtained by shaking, showed the highest content of total polyphenols, 10.04 ± 0.29 mgGAE/g. In the case of the ethanolic extract obtained by stirring, the highest percentage of inhibition was obtained, with a value of 48.71%. After calculating the final results in relation to the reference curve performed on the routine standard, the values varied from 2001.8 ± 1.6 ?g/ml, respectively 2.0018 mg/ml for the extract of bilberries in ethanol, up to the value the highest of 3105.4 ± 2.8 ?g/ml, i.e. 3.1054 mg/ml in the case of the methanolic extract.
[1] Al-Matani, Sheikha Khamis, Ruqaiya Nasser Said Al-Wahaibi, Mohammed Amzad Hossain, 2015, Total Flavonoids Content and Antimicrobial Activity of Crude Extract from Leaves of Ficus Sycomorus Native to Sultanate of Oman, Karbala International Journal of Modern Science 1 (3): 166–71
[2] Aryal, Sushant, Manoj Kumar Baniya, Krisha Danekhu, Puspa Kunwar, Roshani Gurung, and Niranjan Koirala, 2019, Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal, Plants 8 (4)
[3] Bayazid, A.B.; Chun, E.M.; Mijan, M.A.; Park, S.H.; Moon, S.-K.; Lim, B.O. Anthocyanins profiling of bilberry (Vaccinium myrtillus L.) extract that elucidates antioxidant and anti-inflammatory effects. Food Agric. Immun. 2021, 32, 713–726.
[4] Celik, F.; Bozhuyuk, M.R.; Ercisli, S.; Gundogdu, M. Physicochemical and Bioactive Characteristics of Wild Grown Bilberry (Vaccinium myrtillus L.) Genotypes from Northeastern Turkey. Not. Bot. Horti Agrobot. Cluj-Napoca 2017, 46, 128–133.
[5] Cote J, Caillet S, Doyon G, Sylvain JF, Lacroix M (2010) Bioactive compounds in cranberries and their biological properties. Crit Rev Food Sci Nutr 50:666–679. doi:10.1080/10408390903044107
[6] Chen L. Y., Cheng C. W., Liang J. Y, 2015, Effect of esterification condensation on the Folin–Ciocalteu method for the quantitative measurement of total phenols, Food Chemistry, vol. 170, pp. 10–15, citated by Carmona-Hernandez et al., 2021
[7] Estrada AER, Lee HJ, Beelman RB, del Mar Jimenez-Gasco M, Royse DJ (2009) Enhancement of the antioxidants ergothioneine and selenium in Pleurotus eryngii var. eryngii basidiomata through cultural practices. World J Microbiol Biotechnol 25:1597– 1607. doi:10.1007/s11274-009-0049-8
[8] Karcheva-Bahchevanska, D.; Lukova, P.; Nikolova, M.M.; Mladenov, R.D.; Iliev, I.N. Effect of Extracts of Bilberries (Vaccinium myrtillus L.) on Amyloglucosidase and ?-Glucosidase Activity. Folia Med. 2017, 59, 197–202.
[9] Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud 15:523–530
[10] Nestby, R.; Percival, D.; Martinussen, I.; Opstad, N.; Rohloff, J. The european blueberry (Vaccinium myrtillus L.) and the potential for cultivation. A review. Eur. J. Plant Sci. Biotech. 2011, 5, 5–16
[11] Newell AM, Yousef GG, Lila MA, Ramirez-Mares MV, de Mejia EG (2010) Comparative in vitro bioactivities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells. J Ethnopharmacol 130:536–544. doi:10.1016/j.jep.2010.05.051
[12] Pekal, A., Pyrzynska K., 2014., Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay, Food Analytical Methods 7 (9): 1776–82
[13] Rusak G., Komes D., Likic? S., Horz?ic? D., Kovac? M., 2008, Phenolic content and antioxidative capacity of green and white tea extracts depending on extraction conditions and the solvent used, Food Chemistry, vol. 110, no. 4, pp. 852–858, 2008, citat de Carmona-Hernandez et al., 2021
[14] Sa?nchez-Rangel J. C., Benavides J., Heredia J. B, Cisneros- Zevallos L., Jacobo- Vela?zquez D.A., 2013, The Folin–Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination, Analytical Methods, vol. 5, no. 21, pp. 5990–5999, citat de Carmona-Hernandez et al., 2021
[15] Sies, H. ,2007, Total antioxidant capacity: appraisal of a concept, The Journal of nutrition 137.6:1493-1495
[16] ***Ocolul Silvic Teregova, Amenajamentul U.P. V Higigel
All authors are main authors, have equal rights and have contributed evenly to this paper.
conference
Proceedings of 23rd International Multidisciplinary Scientific GeoConference SGEM 2023
23rd International Multidisciplinary Scientific GeoConference SGEM 2023, 03 - 09 July, 2023
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
351-358
03 - 09 July, 2023
website
9166
antioxidant activity; flavonoid content; phenolic content; bilberries