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Title: ANTICANCER AND ANTIBACTERIAL POTENTIAL OF METHOTREXATE LOADED IRON NANOPARTICLES PRODUCED BY GREEN SYNTHESIS METHOD
ANTICANCER AND ANTIBACTERIAL POTENTIAL OF METHOTREXATE LOADED IRON NANOPARTICLES PRODUCED BY GREEN SYNTHESIS METHOD

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Tsvetelina Batsalova; Dzhemal Moten; Anastasiia Voronova; Balik Dzhambazov; Alexander Vasil’kov
10.5593/sgem2023/6.1/s24.01
10.5593/sgem2023/6.1
1314-2704
978-619-7603-61-3
English
23
6.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Micro and Nano Technologies
An environmentally safe method – metal-vapor synthesis (MVS), was applied to produce iron nanoparticles (Fe NPs), which were conjugated with the drug methotrexate (MTX). The physicochemical properties and biological activity of the generated Fe NPs-MTX nanomaterials were analyzed. Transmission electron microscopy observations indicated that Fe NPs are stabilized in the form of aggregates with a size of 100 nm. The structure of these aggregates is similar to a “bunch of grapes”, which consists of metal particles about 1.83 nm in size. X-ray photoelectron spectroscopy evaluations showed that Fe both in the black and the composite with methotrexate was in Fe 3+ state, and the Fe 2+ state was also present in a small amount. Biological activity of Fe NPs-MTX conjugates against bacterial and human cells was assessed. Our results demonstrated inhibitory effects against both Gram-positive and Gram-negative bacteria that were superior compared to the activity of unloaded nanoparticles. Analyses with human cell lines showed time- and concentration-dependent cytotoxicity of Fe NPs-MTX against cancer cells and low inhibitory activity towards normal fibroblasts proving the anticancer potential of the nanoconjugates. In addition, colon adenocarcinoma-specific effect was observed based on the detection of highest inhibitory effects of Fe NPs-MTX against HT29 cell line. In vitro assays initially demonstrated lysosome-directed toxicity which after a longer exposition period (72-120h) was superseded by pronounced inhibition of cellular metabolic activity. These data indicate a wide biological functionality spectrum of MVS produced Fe NPs loaded with MTX and their potential for biomedical application.
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The research was supported by the National Science Foundation of Bulgaria (project number KP-06-RUSSIA-14), Russian Foundation for Basic Research (project number 20- 53-18006), Ministry of Science and Higher Education of the Russian Federation (Contract No. 075-03-2023-642) and was performed employing the equipment of Center for molecular composition studies of INEOS RAS.
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https://doi.org/10.5593/sgem2023/6.1/s24.01
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Proceedings of 23rd International Multidisciplinary Scientific GeoConference SGEM 2023
23rd International Multidisciplinary Scientific GeoConference SGEM 2023, 03 - 09 July, 2023
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
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03 - 09 July, 2023
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anticancer activity, biological evaluation, iron nanoparticles, metal-vapor synthesis, methotrexate