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Title: ANTIMICROBIAL RESISTANCE AND BIOFILM FORMATION OF SOME STAPHYLOCOCCUS SPECIES ISOLATED FROM TRADITIONAL CHEESES

ANTIMICROBIAL RESISTANCE AND BIOFILM FORMATION OF SOME STAPHYLOCOCCUS SPECIES ISOLATED FROM TRADITIONAL CHEESES

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Alex-Cristian Moza; Emil Tirziu; Ileana Nichita; Iulia Maria Bucur; Diana Obi?tioiu
10.5593/sgem2023/6.1/s25.16
10.5593/sgem2023/6.1
1314-2704
978-619-7603-61-3
English
23
6.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
This research aimed to determine the occurrence of different Staphylococcus spp., in three types of Romanian traditional cheeses made of raw milk, and to establish their antimicrobial resistance profile. Additionally, we have also screened the biofilm forming capacity of the S. aureus isolates. Thus, 25 samples (cow telemea n = 10, sheep telemea n = 5, and cow ca? n = 10) were collected from local small producers that sell in urban fresh markets in Timi? county. A total of 64% (16/25) of the examined samples were positive. The bacterial strains were isolated on Mannitol Salt Agar (MSA) medium, then Baird-Parker medium was used to emphasize the coagulase activity. Furthermore, using the Vitek 2 Compact equipment, 17 strains were identified and included in 4 species of Staphylococcus genus, respectively one coagulase-positive (CPS) represented by Staphylococcus aureus 52.97% (9/17), and 3 coagulase-negative (CNS) comprised of S. epidermidis 23.52% (4/17), S. chromogenes 11.76% (2/17) and S. saprophyticus 11.76% (2/17). Disc diffusion method showed that the S. aureus isolates presented more resistance towards penicillin (100%), erythromycin, oxacillin, gentamicin, tetracycline (66.66%), and ciprofloxacin, clindamycin (55.55%). CNS isolates were more resistant towards penicillin (83.33%), erythromycin (66.66%), oxacillin (50), and tetracyclin (41.66%). From the total of 9 S. aureus, all were able to produce biofilms with absorbance at 570 nm.
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conference
https://doi.org/10.5593/sgem2023/6.1/s25.16
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Proceedings of 23rd International Multidisciplinary Scientific GeoConference SGEM 2023
23rd International Multidisciplinary Scientific GeoConference SGEM 2023, 03 - 09 July, 2023
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
137-144
03 - 09 July, 2023
website
9228
Staphylococcus aureus, traditional cheese, multidrug resistance, biofilm