Peer-reviewed articles 17,970 +


Title: SUPPRESSION OF FUNGAL PHYTOPATHOGENS (FUSARIUM SPP AND ALTERNARIA SPP) ON THE TOMATO LEAVES BY THE RHAMNOLIPIDS PRODUCED BY PSEUDOMONAS AERUGINOSA PAO1
SUPPRESSION OF FUNGAL PHYTOPATHOGENS (FUSARIUM SPP AND ALTERNARIA SPP) ON THE TOMATO LEAVES BY THE RHAMNOLIPIDS PRODUCED BY PSEUDOMONAS AERUGINOSA PAO1

PlumX Article Metrics by Elsevier
Gulnaz Galieva; Guliua Sageeva; Aleksander Gordeev; Polina Kuryntseva; Polina Galitskaya
10.5593/sgem2023/6.1/s25.28
10.5593/sgem2023/6.1
1314-2704
978-619-7603-61-3
English
23
6.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
Suppression of fungal phytopathogens is one of the serious challenges agriculture and forestry since they cause significant biomass and yield losses. One of the alternative methods of suppression phytopathogens is the use of biosurfactants, which are amphiphilic substances produced by many microbes. Bacteria of Pseudomonas genus are known to produce biosurfactants of the rhamnolipid class. These surfactants can possess antimicrobial and fungicidal properties.
In this work, we studied the effect of various concentrations of rhamnolipid (100, 500, 1000 mg/l), produced by Pseudomonas aeruginosa PAO1 strain on the growth of fungal pathogens Fusarium oxysporum and Alternaria spp on tomato leaves that were a) separated from the plants and b) not separated from the plants.
On infected separated leaves without biosurfactant treatment, the area covered by Alternaria spp and F. oxysporum was 28% and 8%, respectively. The growth of Alternaria spp was fully inhibited when the leaf spayed with 1000 mg/l rhamnolipid before artificial infection, and of F. oxysporum – with 100 mg/l rhamnolipid. On unseparated biosurfactants-untreated leaves that remained growing on the tomato plants after artificial infection with Alternaria spp and F. oxysporum was 4.7 and 4.2 scores, respectively. Infection of the leaves did not lead to infection of the whole plants since infected leaves were defoliated. Interestingly, biosurfactants treatment led to growth stimulation of tomato plants at a concentration of 1000 mg/l.
It can be concluded that rhamnolipid produced by P. aeruginosa PAO1 is a promising biocontrol agent.
[1] Bernardes M.F.F., Pazin M., Dorta L.C.P, Impact of Pesticides on Environmental and Human Health. Toxicology Studies - Cells, Drugs and Environment, 2015.
[2] Bonner M. R., Alavanja M. C. R, Pesticides, human health, and food security, Food and Energy Security, vol.6, pp 89–93, 2017.
[3] Koskey G., Mburu S. W., Awino R., Njeru E. M., Maingi J. M, Potential Use of Beneficial Microorganisms for Soil Amelioration, Phytopathogen Biocontrol, and Sustainable Crop Production in Smallholder Agroecosystems, Frontiers in Sustainable Food Systems, vol.5, pp 130, 2021.
[4] Batista B. D., Singh B. K, Realities and hopes in the application of microbial tools in agriculture, Microbial Biotechnology, vol.14, pp 1258, 2021.
[5] Robineau M., Le Guenic S., Sanchez L., et al, Synthetic Mono-Rhamnolipids Display Direct Antifungal Effects and Trigger an Innate Immune Response in Tomato against Botrytis Cinerea, Molecules 2020, vol.25, pp 3108, 2020.
[6] Monnier N., Cordier M., Dahi A., et al, Semipurified rhamnolipid mixes protect Brassica napus against Leptosphaeria maculans early infections, Phytopathology, vol.110, pp 834–842, 2020.
[7] Thakur P., Saini N.K., Thakur V.K., Gupta V.K., Saini R. V., Saini A.K, Rhamnolipid the Glycolipid Biosurfactant: Emerging trends and promising strategies in the field of biotechnology and biomedicine, Microbial Cell Factories, vol.20, pp 1, 2021.
[8] Sachdev D. P., Cameotra S. S, Biosurfactants in agriculture, Applied Microbiology and Biotechnology, vol.97, pp 1005, 2013.
[9] Goswami D., Borah S. N., Lahkar J., Handique P. J., Deka S, Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum, Journal of Basic Microbiology, vol.55, pp 1265–1274, 2015.
[10] Biktasheva L., Gordeev A., Selivanovskaya S., Galitskaya P, Di- and Mono- Rhamnolipids Produced by the Pseudomonas putida PP021 Isolate Significantly Enhance the Degree of Recovery of Heavy Oil from the Romashkino Oil Field (Tatarstan, Russia), Processes 2022, Vol. 10, Page 779, vol.10, pp 779, 2022.
[11] Scheff S. W, Nonparametric Statistics, Fundamental Statistical Principles for the Neurobiologist, pp 157–182, 2016 doi:10.1016/B978-0-12-804753-8.00008-7.
[12] Sampaio A. M. et al. A diversity of resistance sources to Fusarium oxysporum f. sp. pisi found within grass pea germplasm, Plant and Soil, vol.463, pp 19–38, 2021.
[13] Sampaio A.M., Vitale S., Turra D., et al, Alternaria spp.: from general saprophyte to specific parasite, Molecular Plant Pathology, vol.4, pp 225–236, 2003.
[14] Manjunatha N., Sharma J., Pokhare S.S., et al, Characterization of Alternaria and Colletotrichum Species Associated with Pomegranate (Punica granatum L.) in Maharashtra State of India, Journal of Fungi, vol.8, pp 1040, 2022.
[15] Leiminger J. H., Auinger H. J., Wenig M., Bahnweg G., Hausladen H, Genetic variability among Alternaria solani isolates from potatoes in Southern Germany based on RAPD-profiles, Journal of Plant Diseases and Protection, vol.120, pp.164-172, 2013.
The work is carried out in accordance with the Strategic Academic Leadership Program «Priority 2030» of the Kazan Federal University of the Government of the Russian Federation.
conference
https://doi.org/10.5593/sgem2023/6.1/s25.28
Download PDF
Proceedings of 23rd International Multidisciplinary Scientific GeoConference SGEM 2023
23rd International Multidisciplinary Scientific GeoConference SGEM 2023, 03 - 09 July, 2023
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Turkish Acad Sci.
225-232
03 - 09 July, 2023
website
9240
Biosurfactant, rhamnolipid, phytopathogen, Fusarium oxysporum and Alternaria spp, biocontrol agent