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Title: ENHANCING THE ANTIOXIDANT ACTIVITY OF CHAMOMILE: EXPLORING THE IMPACT OF ULTRASOUND ON PROTEIN EXTRACTION

ENHANCING THE ANTIOXIDANT ACTIVITY OF CHAMOMILE: EXPLORING THE IMPACT OF ULTRASOUND ON PROTEIN EXTRACTION

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Ancuta Lupaescu; Nicoleta Flutur; Brindusa Alina Petre; Monica Iavorschi; Florin Ursachi
10.5593/sgem2023V/6.2/s25.20
10.5593/sgem2023v/6.2
1314-2704
978-619-7603-66-8
English
23
6.2
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
Chamomile flower, a well-known medicinal plant, possesses significant antioxidant properties attributed to its diverse array of bioactive compounds, including antioxidant proteins. These proteins are vital in combating oxidative stress and neutralizing harmful free radicals. To fully harness the antioxidant potential of chamomile, it is imperative to optimize the extraction methods. This study evaluates the effectiveness of nonconventional mechanical extraction methods using ultrasound to extract antioxidant compounds from chamomile, comparing it with a traditional agitation-based technique. The extracts obtained using green solvents such as basic aqueous solutions or phosphate-buffered saline (PBS) showed that while the ultrasound extraction requires a shorted extraction time compared to traditional methods, it yields a more active extract in terms of antioxidant activity. These active constituent-rich extracts were then subjected to thorough characterization and quantification using advanced analytical techniques, such as gel electrophoresis, mass spectrometry, UV-vis, and FTIR spectroscopy. Finally, the antioxidant capacity of the aqueous extract was determined using the DPPH free radical method and the FRAP assay.
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This work was funded by Ministry of Research, Innovation and Digitalization withinProgram 1 - Development of national research and development system, Subprogram1.2 - Institutional Performance - RDI excellence funding projects, under contract no.10PFE/2021
conference
https://doi.org/10.5593/sgem2023V/6.2/s25.20
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Proceedings of 23rd International Multidisciplinary Scientific GeoConference SGEM 2023
23rd International Multidisciplinary Scientific GeoConference SGEM 2023, 28-30 November, 2023
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference-SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
157-164
28-30 November, 2023
website
9596
Chamomile, antioxidant activity, proteins, ultrasound extraction.