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Title: THUJA OCCIDENTALIS AND PLATYCLADUS ORIENTALIS ANTIMICROBIAL ACTIVITY
THUJA OCCIDENTALIS AND PLATYCLADUS ORIENTALIS ANTIMICROBIAL ACTIVITY

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Doris Floares; Diana Obistioiu; Anca Hulea; Ersilia Alexa; Isidora Radulov
10.5593/sgem2023V/6.2/s25.57
10.5593/sgem2023v/6.2
1314-2704
978-619-7603-66-8
English
23
6.2
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
The rise of bacterial resistance to currently employed antibiotics is causing growing concerns for public health. The emergence of highly resistant bacterial strains results in the ineffectiveness of antibiotic treatments against many bacterial infections. As a result, there is an ongoing quest for new antimicrobial agents. This pursuit can take two main directions: one involves the design and synthesis of novel agents, while the other involves exploring natural sources to uncover previously undiscovered antimicrobial compounds. Herbal medications, particularly, have garnered renewed interest due to the perception that they tend to cause fewer adverse reactions when compared to synthetic pharmaceuticals. Moreover, the lower costs of producing plant-based preparations make searching for natural therapeutics appealing. This study aims to assess the antimicrobial properties of Thuja occidentalis (TO) and Platycladus orientalis (PO) against Gram-positive and Gram-negative bacteria using specified reference strains: Streptococcus pyogenes (ATCC 19615), Staphylococcus aureus (ATCC 25923), Listeria monocytogenes (ATCC 19114), Bacillus cereus (ATCC 10876), Clostridium perfringens (ATCC 13124), Shigella flexneri (ATCC 12022), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Salmonella typhimurium (ATCC 14028) and Haemophilus influenzae type B (ATCC 10211) Additionally, we determined the minimum inhibitory concentration (MIC). Following ISO 20776-1:2019 guidelines, we assessed the antimicrobial activity by measuring the reduction in microbial mass through spectrophotometry to determine changes in optical density (OD). Our findings indicate that the TO and PO extracts inhibit Gram-positive bacteria, particularly at the initial concentration tested.
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[5] Darwish, R.S., Hammoda, H.M., Ghareeb, D.A., Abdelhamid, A.S.A., Harraz,F.M., & Shawky, E. Seasonal dynamics of the phenolic constituents of the cones andleaves of oriental Thuja (Platycladus orientalis L.) reveal their anti-inflammatorybiomarkers. RSC advances, 11(40), 24624–24635. https://doi.org/10.1039/d1ra01681d,2021
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[12] Khammassi, M., Ben Ayed, R., Khedhri, S., Souihi, M., Hanana, M., Amri, I.,Hamrouni, L. Crude extracts and essential oil of Platycladus orientalis (L.) Franco: asource of phenolics with antioxidant and antibacterial potential as assessed through achemometric approach, Turkish Journal of Agriculture and Forestry:46:477-487doi:10.55730/1300-011X.3019, 2022
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We have performed this research with the support of the Interdisciplinary ResearchPlatform belonging to the University of Life Sciences “King Michael I” fromTimisoara, where the analysis were made.Support was also received by the project Horizon Europe (HORIZON) 101071300 -Sustainable Horizons -European Universities designing the horizons ofsustainability (SHEs)
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https://doi.org/10.5593/sgem2023V/6.2/s25.57
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Proceedings of 23rd International Multidisciplinary Scientific GeoConference SGEM 2023
23rd International Multidisciplinary Scientific GeoConference SGEM 2023, 28-30 November, 2023
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference-SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
465-472
28-30 November, 2023
website
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Gram-positive, Gram-negative, MIC

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