Peer-reviewed articles 17,970 +


Luminita Costinar; Viorel Herman; Corina Pascu
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
The golden jackal (Canis aureus), a mesocarnivore, is currently spreading from eastern to western Europe. In Europe, the golden jackal is considered an invasive species, as in recent years it has managed to populate extensive territories in Greece, Turkey, Slovenia, Serbia and Hungary. Bulgaria is considered the country with the largest population of jackals in Europe. In Romania it is frequently found from the plains of Dobrogea to Banat. The present work aimed to study and observe the newly developed antibiotic resistance profiles of intestinal bacterial flora isolated from these wild species and to compare our data with those existing in the literature. Duodenum samples from golden jackals were collected for primary bacteriological examinations. This study used common identification and isolation methods and modern identification techniques, such as proteomics and MALDI-TOF MS technique, for highly accurate information on the bacterial flora of the digestive system. Antibiograms were performed using the disc-diffusimetric method using antibiotics and chemotherapeutics belonging to several groups. Several bacterial species were isolated from the jackals studied: Serratia liquefaciens, Staphylococcus hominis and Aeromonas salmonicida. In Aeromonas salmonicida strains, antibiograms showed significant percentages of intermediate strains to most of the antimicrobials used and overall resistance to levofloxacin. The same behaviour was recorded for colistin and strains belonging to the Seratia and Streptococcus genera. Interestingly, some bacterial strains isolated showed resistance to levofloxacin, a third- generation fluoroquinolone, as this antimicrobial is banned for veterinary use in EU countries. The results are a wake-up call, as antibiotic resistance has been shown to be present in wild animal populations in Romania, with risk to public health.
[1] Abbasi-Montazeri, E., Khodavaisy, S., Serratia marcescens, an emerging nosocomialpathogen: tracking the pathogenicity factors, Antibiotics, 2020, 9(9), 623;
[2] Albrechtova K, Papousek I, De Nys H, Pauly M, et all., , Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Tai National Park, Cote d'Ivoire, surroundedby villages with high prevalence of multiresistant ESBL-producing Escherichia coli inpeople and domestic animals. PLoS One. (2014), Dec 4;9(12):e113548;
[3] Costinar, L., Herman, V., Iancu, I., Pascu, C., Phenotypic characterizations andantimicrobials resistance of Salmonella strains isolated from pigs from fattening farms,Rev. Rom. Med. Vet., 2021, 31 | 2: 31-34;
[4] Costinar, L., Herman, V., Pitoiu, E., Iancu, I., Degi, J., Hulea, A., Pascu, C. BoarSemen Contamination: Identification of Gram-Negative Bacteria and AntimicrobialResistance Profile. Animals, 2022, 12, 43;
[5] Kemenszky, P., Janoska, F., Nagy, G., Csivincsik, A. The golden jackal (Canis aureus)and the African swine fever pandemic: Its role is controversial but not negligible (a dietanalysis study). Vet. Med. Sci. 2022, 8, 97–103;
[6] Herman, V., Pascu, C., Costinar, L., Faur, B., Vaduva, I., E. coli strainscharacterization isolated from pig septicemic colibacilosis, Lucrari Stiintifice MedicinaVeterinara, 2010, 43, 1, 93-96;
[7] Yousuf, M.A., Bashu, J., Pervin, M., Islam, M.T., Das, P.M., Khan, M.A.H.N.A.Identifying diseases of golden jackals of Bangladesh Agricultural University campus,Mymensingh, Bangladesh. Bangl. J. Vet. Med. 2014, 12, 217–224;
[8] Martinez, J.L., Antibiotics and antibiotic resistance genes in natural environments.Science, 2012, 321(5897), 365-367;
[9] Pascu, C., Herman, V., Iancu, I., Costinar, L. Etiology of Mastitis and AntimicrobialResistance in Dairy Cattle Farms in the Western Part of Romania, Antibiotics, 2022, 11,57;
[10] Pascu, C., Herman, V., Costinar, L., Iancu, I., Antimicrobial susceptibility ofpathogenic bacteria isolated from swine lungs. Rom. Biotechnol. Lett. 2019, 24, 506–512;
[11] Traub, W.H., Antibiotic susceptibility of Serratia marcescens and Serratialiquefaciens. Chemotherapy. 2000, Sep-Oct;46(5):315-21;
[12] Di Francesco, A., Salvatore, D., Gobbi, M. et al. Antimicrobial resistance genes in agolden jackal (Canis aureus L. 1758) from Central Italy. Vet Res Commun, 2023,;
[13] Stock, I., Grueger, T., Wiedemann, B., Natural antibiotic susceptibility of strains ofSerratia marcescens and the S. liquefaciens complex: S. liquefaciens sensu stricto, S.proteamaculans and S. grimesii. Int J Antimicrob Agents., 2003, Jul;22(1):35-47. doi:10.1016/s0924-8579(02)00163-2. PMID: 12842326;
[14] Castanheira, M., Simner, J., Bradford, P.A., Extended-spectrum ?-lactamases: anupdate on their characteristics, epidemiology and detection. 2021, JAC-AMR3:dlab092.;
[15] Valiakos, G., Kapna, I., Colistin resistant mcr genes prevalence in livestock animals(swine, bovine, poultry) from a multinational perspective. A systematic review. Vet Sci2021, 8:265;
[16] Dias,. D, Hipolito, D., Figueiredo, A., Fonseca, C., Caetano, T., Mendo, S.,Unravelling the diversity and abundance of the red fox (Vulpes vulpes) faecal resistomeand the phenotypic antibiotic susceptibility of indicator bacteria, Animals, 2022, 12:2572;
[17] Markov, G., Kocheva, M., Gospodinova, M., Assessment of heavy metalaccumulation in the golden jackal (Canis aureus) as a possible bioindicator in anagricultural environment in Bulgaria, Bull. Environ. Contam. Toxicol., 2016, 96(4):458–464;
[18] Plaza-Rodriguez, C., Alt, K., Grobbel, M., Hammerl, J.A., Irrgang, A., Szabo, I.,Stingl, K., Schuh, E., Wiehle, L., Pfefferkorn, B., Naumann, S., Kaesbohrer, A.,Tenhagen BA Wildlife as sentinels of antimicrobial resistance in Germany? Front VetSci, 2021, 7:627821;
Proceedings of 23rd International Multidisciplinary Scientific GeoConference SGEM 2023
23rd International Multidisciplinary Scientific GeoConference SGEM 2023, 28-30 November, 2023
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference-SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
28-30 November, 2023
antibiotic resistance, golden jackal, Serratia spp., Staphylococcus spp., Aeromonas spp., MALDI-TOF Ms