Peer-reviewed articles 17,970 +



Title: EFFECT OF ZINC OXIDE NANOPARTICLES AGAINST PHYTOPHTHORA SPP. CAUSING DURIAN DISEASES IN VITRO

EFFECT OF ZINC OXIDE NANOPARTICLES AGAINST PHYTOPHTHORA SPP. CAUSING DURIAN DISEASES IN VITRO
Kanchalar Keeratirawee; Pornprapa Kongtragoul; Sansanee Pantakan
10.5593/sgem2024/6.1
1314-2704
English
24
6.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
The efficiency of zinc oxide nanoparticles (ZnO-NPs) in inhibiting Phytophthora spp. growth in both durian fruits and stems is demonstrated. The size of ZnO-NPs used was in range of 25-50 nm in diameter. Diseased durian sample were collected from a durian orchard located in southern of Thailand. The pathogen of diseased durian was isolated for subsequent morphological identification. Three distinct strains of Phytophthora spp. (designated as PHY41, PHY45, PHY49) were successfully isolated and were found in colonies with rings and stellate pattern, ovoid, limoniform sporangium with semi-papillate or papillate, and formation chlamydospores. All isolates were exhibiting 100% pathogenicity on durian, as indicated by the disease incidence (DI). The concentration of ZnO-NPs played a crucial role in suppressing the growth of Phytophthora spp. in all isolates. Higher concentration of ZnO-NPs resulted in increased inhibition of growth. ZnO-NPs at the concentration of 2000 ?g/mL effectively suppressed the mycelial growth of all fungi isolates at 57.76-69.84%. Additionally, ZnO-NPs at the concentration of 500 ?g/mL completely inhibited the sporulation only in the PHY49 strains. Furthermore, ZnO-NPs concentration ranging from 1000-2000 ?g/mL resulted in completed inhibition in sporulation in all Phytophthora spp. isolates. The result demonstrated that ZnO-NPs at all concentrations effectively delayed the germination of Phytophthora spp.
[1] C. R. Mendes et al., "Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens," Scientific Reports, vol. 12, no. 1, p. 2658, 2022/02/16 2022, doi: 10.1038/s41598-022-06657-y.
[2] A. Miri, N. Mahdinejad, O. Ebrahimy, M. Khatami, and M. Sarani, "Zinc oxide nanoparticles: Biosynthesis, characterization, antifungal and cytotoxic activity," Materials Science and Engineering: C, vol. 104, p. 109981, 2019/11/01/ 2019, doi: https://doi.org/10.1016/j.msec.2019.109981.
[3] S. Ritmontree and P. Kongtragoul, "Antifungal effect of zinc oxide nanoparticles against disease in durian caused by Phytophthora palmivora," 2021.
[4] P. Kongtragoul, K. Ishikawa, and H. Ishii, "Metalaxyl Resistance of Phytophthora palmivora Causing Durian Diseases in Thailand," Horticulturae, vol. 7, no. 10, p. 375, 2021. [Online]. Available: https://www.mdpi.com/2311-7524/7/10/375.
[5] L. T. KWEE, N. C. CHONG, and C. C. LAN, "Etiology and control of durian foliar blight and dieback caused by Rhizoctonia solani," Annals of Applied Biology, vol. 111, no. 2, pp. 301-307, 1987, doi: https://doi.org/10.1111/j.1744-7348.1987.tb01456.x.
[6] T. Lim and S. Sangchote, "Diseases of durian," Diseases of tropical fruit crops, pp. 241-251, 2003.
[7] R. Piasai, P. Chalmers, O. Piasai, and N. Khewkhom, "Postharvest fungicide dips to control fruit rot of ‘Monthong’ durian (Durio zibethinus)," European Journal of Plant Pathology, vol. 160, no. 2, pp. 325-336, 2021/06/01 2021, doi: 10.1007/s10658-021-02246-3.
[8] R. Pongpisutta et al., "Comprehensive Investigation of Die-Back Disease Caused by Fusarium in Durian," Plants, vol. 12, no. 17, p. 3045, 2023. [Online]. Available: https://www.mdpi.com/2223-7747/12/17/3045.
[9] M. E. Gallegly and C. Hong, Phytophthora: identifying species by morphology and DNA fingerprints. APS Press, 2008.
[10] G. Wagner, V. D. Korenkov, J. D. Judy, and P. M. Bertsch, "Nanoparticles Composed of Zn and ZnO Inhibit Peronospora tabacina Spore Germination in vitro and P. tabacina Infectivity on Tobacco Leaves," Nanomaterials, vol. 6, 2016.
[11] P. A. Arciniegas-Grijalba, M. C. Patino-Portela, L. P. Mosquera-Sanchez, J. A. Guerrero-Vargas, and J. E. Rodriguez-Paez, "ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor," Applied Nanoscience, vol. 7, no. 5, pp. 225-241, 2017/06/01 2017, doi: 10.1007/s13204-017-0561-3.
The authors sincerely thank King Mongkut's Institute of Technology Ladkrabang, Prince of Chumphon Campus, Chumphon, Thailand, for support.
conference
Proceedings of 24th International Multidisciplinary Scientific GeoConference SGEM 2024
24th International Multidisciplinary Scientific GeoConference SGEM 2024, 1 - 7 July, 2024
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
51-58
1 - 7 July, 2024
website
9782
Durian diseases, Pathogenicity test, Phytophthora spp., ZnO-NPs, Inhibit

25th SGEM International Conference on Earth & Planetary Sciences


International GeoConference SGEM2025
27 June - 6 July, 2025 / Albena, Bulgaria

Read More
   

SGEM Vienna GREEN "Green Science for Green Life"


Extended Scientific Sessions SGEM Vienna GREEN
3 -6 December, 2025 / Vienna, Austria

Read More
   

A scientific platform for Art-Inspired Scientists!


The Magical World Where Science meets Art
Vienna, Austria

Read More