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EFFECT OF ZINC OXIDE NANOPARTICLES AGAINST PHYTOPHTHORA SPP. CAUSING DURIAN DISEASES IN VITRO
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Kanchalar Keeratirawee; Pornprapa Kongtragoul; Sansanee Pantakan
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10.5593/sgem2024/6.1
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1314-2704
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English
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24
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6.1
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• Prof. DSc. Oleksandr Trofymchuk, UKRAINE
• Prof. Dr. hab. oec. Baiba Rivza, LATVIA |
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The efficiency of zinc oxide nanoparticles (ZnO-NPs) in inhibiting Phytophthora spp. growth in both durian fruits and stems is demonstrated. The size of ZnO-NPs used was in range of 25-50 nm in diameter. Diseased durian sample were collected from a durian orchard located in southern of Thailand. The pathogen of diseased durian was isolated for subsequent morphological identification. Three distinct strains of Phytophthora spp. (designated as PHY41, PHY45, PHY49) were successfully isolated and were found in colonies with rings and stellate pattern, ovoid, limoniform sporangium with semi-papillate or papillate, and formation chlamydospores. All isolates were exhibiting 100% pathogenicity on durian, as indicated by the disease incidence (DI). The concentration of ZnO-NPs played a crucial role in suppressing the growth of Phytophthora spp. in all isolates. Higher concentration of ZnO-NPs resulted in increased inhibition of growth. ZnO-NPs at the concentration of 2000 ?g/mL effectively suppressed the mycelial growth of all fungi isolates at 57.76-69.84%. Additionally, ZnO-NPs at the concentration of 500 ?g/mL completely inhibited the sporulation only in the PHY49 strains. Furthermore, ZnO-NPs concentration ranging from 1000-2000 ?g/mL resulted in completed inhibition in sporulation in all Phytophthora spp. isolates. The result demonstrated that ZnO-NPs at all concentrations effectively delayed the germination of Phytophthora spp.
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conference
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Proceedings of 24th International Multidisciplinary Scientific GeoConference SGEM 2024
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24th International Multidisciplinary Scientific GeoConference SGEM 2024, 1 - 7 July, 2024
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Proceedings Paper
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STEF92 Technology
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International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM
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SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
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51-58
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1 - 7 July, 2024
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website
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9782
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Durian diseases, Pathogenicity test, Phytophthora spp., ZnO-NPs, Inhibit
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