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Title: COMPARATIVE STUDY ON PHENOLICS, FLAVONOIDS, TANNINS AND ANTIOXIDANT PROPERTIES OF CALENDULA OFFICINALIS EXTRACTS OBTAINED BY DIFFERENT EXTRACTION TECHNIQUES
COMPARATIVE STUDY ON PHENOLICS, FLAVONOIDS, TANNINS AND ANTIOXIDANT PROPERTIES OF CALENDULA OFFICINALIS EXTRACTS OBTAINED BY DIFFERENT EXTRACTION TECHNIQUES

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Lucia-Florina Popovici; Simona Oancea
10.5593/sgem2024/6.1/s25.21
10.5593/sgem2024/6.1
1314-2704
978-619-7603-74-3
English
24
6.1
•    Prof. DSc. Oleksandr Trofymchuk, UKRAINE 
•    Prof. Dr. hab. oec. Baiba Rivza, LATVIA
Advances in Biotechnology
The aim of this study was to evaluate the influence of several extraction methods on the content of bioactive compounds of polyphenolic structure and antioxidant properties of petals from marigold flowers (Calendula officinalis).
Ethanolic extracts prepared using maceration, turbo-extraction and bead-beating techniques were investigated for total phenolics, flavonoids and tannins, and antioxidant activities as measured by FRAP and DPPH assays, as well.
The results showed that the highest phenolic content (1316.367 ± 4.316 mg GAE/100 g DW) and antioxidant activity by FRAP (505.755 ± 4.623 mg ascorbic acid/100 g DW) were obtained by maceration for 24 h at room temperature, while the highest flavonoid content (2729.521 ± 7.261 mg quercetin /100 g DW) and tannin content (476.748 ± 4.126 mg catechin /100 g DW) were recorded in the sample subjected to turbo-extraction for 5 min. Turbo-extraction proved to be an efficient technique for extraction of total phenolics at an extraction time of 10 min, and for total flavonoids and tannins at an extraction time of 5 min. Extracts obtained by short maceration (5 min, 10 min) showed lower values of phenolic content, flavonoid content and antioxidant activity by FRAP, compared to those of sample subjected to maceration for 24 h. Extracts obtained by bead-beating technique at extraction times of 5 and 10 min indicated the lowest phenolic, tannin and flavonoid contents compared to all the other extraction methods. Ceramic (zirconium oxide) bead-beating technique generated lower contents compared to those of sample obtained using metal (stainless steel) bead-beating technique, except for the antioxidant activity by DPPH, which was found the highest among all investigated samples at an extraction time of 10 min (17.726 ± 0.015 %).
Our results suggest that extraction time is a key factor for the investigated techniques. The ethanolic extracts of Calendula officinalis, in particular using turbo-extraction, showed high phenolic content and ferric reducing antioxidant power, using a short extraction time, properties that increase their potential to be applied as bioactive ingredients.
[1] Shahane K., Kshirsagar M., Tambe S., Jain D., Rout S., Ferreira M. K. M., Mali S., Amin P., Srivastav P. P., Cruz J., Lima R. R., An Updated Review on the Multifaceted Therapeutic Potential of Calendula officinalis L., Pharmaceuticals, Switzerland, vol. 16/ issue 4, pp. 1-21, 2023, DOI: https://doi.org/10.3390/PH16040611
[2] Ak G., Zengin G., Sinan K. I., Mahomoodally M. F., Picot-Allain M. C. N., Cakir O., Bensari S., Yilmaz M. A., Gallo M., Montesano D., A Comparative Bio-Evaluation and Chemical Profiles of Calendula officinalis L. Extracts Prepared via Different Extraction Techniques, Applied Sciences, Switzerland, vol. 10/ issue 17, pp. 1-16, 2020, DOI: https://doi.org/10.3390/APP10175920
[3] Singleton V. L., Rossi, J. A., Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents, American Journal of Enology and Viticulture, U.S.A, vol. 16/ issue 3, pp. 144–158, 1965, DOI: https://doi.org/10.5344/AJEV.1965.16.3.144
[4] Kumar S., Kumar D., Jusha M., Saroha K., Singh N., Vashishta B., Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract, Acta Pharmaceutica, Croatia, vol. 58/ issue 2, pp. 215–221, 2008, DOI: https://doi.org/10.2478/V10007-008-0008-1
[5] Price M. L., Scoyoc S. Van, Butler L. G., A Critical Evaluation of the Vanillin Reaction as an Assay for Tannin in Sorghum Grain, Journal of Agricultural and Food Chemistry, U.S.A, vol. 26/ issue 5, pp. 1214–1218, 1978, DOI: https://doi.org/10.1021/JF60219A031/ASSET/JF60219A031.FP.PNG_V03
[6] Benzie I. F. F., Strain J. J., The Ferric Reducing Ability of Plasma (FRAP) as a Measure of ‘Antioxidant Power’: The FRAP Assay, Analytical Biochemistry, U.S.A, vol. 239/ issue 1, pp. 70–76, 1996, DOI: https://doi.org/10.1006/ABIO.1996.0292
[7] Brand-Williams W., Cuvelier M. E., Berset C., Use of a free radical method to evaluate antioxidant activity, LWT - Food Science and Technology, vol. 28/ issue 1, pp. 25–30, 1995, doi: https://doi.org/10.1016/S0023-6438(95)80008-5
[8] de Souza A. P. T. B., Barni S. T., Ferreira R. A., Couto A. G., Technological Development of Hydroalcoholic Extractive Solutions from Calendula officinalis L. Flowers by using Factorial Design, Latin American Journal of Pharmacy, Argentina, vol. 29/ issue 1, pp. 13–21, 2010, ISSN: 0326-2383 (printed ed.), 2362-3853 (on-line ed.)
[9] Untea A., Lupu A., Saracila M., Panaite T., Comparison of ABTS, DPPH, Phosphomolybdenum assays for estimating antioxidant activity and phenolic compounds in five different plant extracts., Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Animal Science & Biotechnologies, Romania, vol. 75/ issue 2, pp. 110–114, 2018, DOI: https://doi.org/10.15835/buasvmcn-asb
[10] Verma P. K., Raina R., Sultana M., Phytochemical constituents and antioxidant potential in floral extracts of Calendula officinalis Linn. World Journal of Pharmaceutical Research, vol. 3, pp. 2067-2083, 2014, ISSN: 2277-7105.
[11] Givol O., Kornhaber R., Visentin D., Cleary M., Haik J., Harats M., A systematic review of Calendula officinalis extract for wound healing, Wound Repair and Regeneration, vol. 27/ issue 5, pp. 548–561, 2019, DOI: https://doi.org/10.1111/WRR.12737
This research was funded by the Lucian Blaga University of Sibiu (Knowledge Transfer Center) & Hasso Plattner Foundation research grants - grant number LBUS-HPI-ERG-2023-04.
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https://doi.org/10.5593/sgem2024/6.1/s25.21
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Proceedings of 24th International Multidisciplinary Scientific GeoConference SGEM 2024
24th International Multidisciplinary Scientific GeoConference SGEM 2024, 1 - 7 July, 2024
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci and Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts and Letters; Acad Fine Arts Zagreb Croatia; Croatian Acad Sci and Arts; Acad Sci Moldova; Montenegrin Acad Sci and Arts; Georgian Acad Sci; Acad Fine Arts and Design Bratislava; Russian Acad Arts; Turkish Acad Sci.
141-148
1 - 7 July, 2024
website
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Calendula officinalis, extraction, phenolics, flavonoids, tannins, FRAP, DPPH.

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