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JOINT IMMOBILIZATION OF TWO OR MORE ENZYMES ON DIFFERENT CARRIERS
Abstract
Multi-enzymatic cascade reactions, that is, the integration of several biocatalytic transformations occurring simultaneously, offer a wide range of possibilities and new ways to synthesize products with high added value. Such systems improve biocatalytic processes by saving time and reducing waste, while being self-sufficient in terms of requirements for related factors. As a more environmentally friendly and sustainable alternative method for the production of chemicals and biological products increases in importance, cascade reactions can be a very promising approach in this direction, eliminating the short traditional step-by-step synthesis. When using multi-enzyme systems in cascade reactions, local substrate concentration occurs around the second and all subsequent enzymes that enter the system. Such systems are particularly attractive because many commercially available enzymes operate under relatively similar environmental conditions (e.g., pH and temperature). In this work, various types of multi-enzyme systems have been studied using various enzymes, such as, for example, cellulase, peroxidase, glucose oxidase, etc. Various methods of immobilization of several enzymes on a single carrier for the production of gluconic acid and its derivatives are described. Examples of the processing of cellulose-containing raw materials using multi-enzyme systems and methods of enzyme compartmentalization are given. This research is particularly relevant because enzymatic cascade systems provide promising approaches to creating more flexible ways to produce various chemical products at a lower cost.
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