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INVESTIGATION OF ANTIBACTERIAL EFFECT OF CHITOSAN-PVA MATRICES CONTAINED SILVER NANOPARTICLES
Abstract
The present work aimed to investigation of influence molecular weight of chitosan, method of silver nanoparticles producing and immobilization, amount of cross-linking agent on antibacterial activity. Silver was incorporated in the matrix in two ways: 1 пїЅ by the recovery of silver ions from AgNO3 solution; 2 ? by addition to hydrogel the silver nanoparticles, obtained previously by microbiological synthesis. The molecular weight of chitosan shows strong influence on internal structure of matrices and on release of silver nanoparticle. The microbiological tests were carried out on two cultures: Bacillus cereus and Pseudomonas aeruginosa. It was found that when silver had been immobilized by reduction, the resulting matrixes do not have sufficient antibacterial activity. When the silver nanoparticles obtained by microbiological synthesis were immobilized on matrices, they retain their antibacterial activity.
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