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AN IN VITRO STUDY: CITRUS LEMON ESSENTIAL OIL AS AN ALTERNATIVE TREATMENT AGAINST MICROBIAL INFECTIONS

Iuliana Popescu, Diana Obiștioiu, Anca Hulea, Doris Floareș, Isidora Radulov

First published: 2023-12-15https://doi.org/10.5593/sgem2023v/6.2/s25.01View metrics

Abstract

A genuine and urgent need is to prevent and treat bacterial diseases primarily caused by germs that have developed antibiotic resistance. These factors led to several studies focusing on alternate or supplementary antimicrobial methods. With the expectation that, unlike antibiotics, they will be effective without causing resistance, researchers are becoming increasingly interested in antimicrobial substances made from natural resources, such as plant extracts, essential oils, and peptides. The present study investigated the antimicrobial behaviour of Citrus lemon essential oil against S. pyogenes (ATCC 19615), S. aureus (ATCC 25923), P. aeruginosa (ATCC 27853), E. coli (ATCC 25922), S. typhimurium (ATCC 14028), H. influenzae type B (ATCC 10211), C. albicans (ATCC 10231), and C. parapsilopsis (ATCC 22019). The microdilution assay demonstrated good antimicrobial efficacy of the essential oil with variable MIC values depending on the microbial species. The chromatographic analyses by GC-MS were performed because the antimicrobial activity is correlated with the chemical composition. The results revealed 24 compounds, with D-limonene (66.67%) and beta-pinene (12.39%) as the predominant compounds.

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Publication details

Title
AN IN VITRO STUDY: CITRUS LEMON ESSENTIAL OIL AS AN ALTERNATIVE TREATMENT AGAINST MICROBIAL INFECTIONS
Authors
Iuliana Popescu, Diana Obiștioiu, Anca Hulea, Doris Floareș, Isidora Radulov
Proceedings
SGEM International Multidisciplinary Scientific GeoConference- EXPO Proceedings; 23rd SGEM International Multidisciplinary Scientific GeoConference Proceedings 2023, Nano, Bio, Green and Space: Technologies for a Sustainable Future, Vol. 23, Issue 6.2
Publisher
STEF92 Technology
Year
2023
Pages
3-8
SWS Citekey
Popescu20232538
ISSN
1314-2704
ISBN
978-619-7603-66-8
Language
en
Publication type
Conference Paper
Proceedings contents
Open official contents
Keywords
References4
  1. Ben Hsouna, A., Ben Halima, N., Smaoui, S. 2017. Citrus lemon essential oil:chemical composition, antioxidant and antimicrobial activities with its preservative effectagainst Listeria monocytogenes inoculated in minced beef meat. Lipids Health Dis 16,146 DOI: 10.1186/s12944-017-0487-5

  2. Asker, M., El-gengaihi, S.E., Hassan, E.M. 2020 Phytochemical constituents andantibacterial activity of Citrus lemon leaves. Bull Natl Res Cent 44, 194DOI: 10.1186/s42269-020-00446-1

  3. Filipowicz N, Kaminski M, Kurlenda J, Asztemborska M. 2003. Antibacterial andantifungal activity of juniper berry oil and its selected components. PhytotherRes.;17:227�31 DOI: 10.1002/ptr.1110

  4. Cowan MM. 1999. Plant products as antimicrobial agents. Clin MicrobiolRev.12:564�82. DOI: 10.1128/cmr.12.4.564

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