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AN IN VITRO STUDY: CITRUS LEMON ESSENTIAL OIL AS AN ALTERNATIVE TREATMENT AGAINST MICROBIAL INFECTIONS
Abstract
A genuine and urgent need is to prevent and treat bacterial diseases primarily caused by germs that have developed antibiotic resistance. These factors led to several studies focusing on alternate or supplementary antimicrobial methods. With the expectation that, unlike antibiotics, they will be effective without causing resistance, researchers are becoming increasingly interested in antimicrobial substances made from natural resources, such as plant extracts, essential oils, and peptides. The present study investigated the antimicrobial behaviour of Citrus lemon essential oil against S. pyogenes (ATCC 19615), S. aureus (ATCC 25923), P. aeruginosa (ATCC 27853), E. coli (ATCC 25922), S. typhimurium (ATCC 14028), H. influenzae type B (ATCC 10211), C. albicans (ATCC 10231), and C. parapsilopsis (ATCC 22019). The microdilution assay demonstrated good antimicrobial efficacy of the essential oil with variable MIC values depending on the microbial species. The chromatographic analyses by GC-MS were performed because the antimicrobial activity is correlated with the chemical composition. The results revealed 24 compounds, with D-limonene (66.67%) and beta-pinene (12.39%) as the predominant compounds.
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References4
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