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SPECIATION AND QUANTIFICATION OF ARSENIC IN WATER AND SEDIMENT SAMPLES BY HPLC-ICP-MS TECHNIQUE
Abstract
The aim of the study was to develop and validate analytical methods for the detection and quantification of arsenic species (As(III), DMA, MMA and As(V)) in different environmental matrices, including drinking water, surface water, groundwater, and sediments. The experimental tests were performed on a HPLC 1260 Infinity II coupled with an ICP-MS 7850 Agilent. For aqueous matrices, the method was validated within a working range of 10-50 microg/L using an Agilent G3154-65002 guard column and an Agilent G3154-65001 analytical column. The mobile phase consisted of a 1 M NaH2PO4 solution with 0.1 mM EDTA-2Na, adjusted to pH 6, and operated at a flow rate of 1.0 mL/min. Method validation yielded limits of quantification ranging from 1.6 microg/L (As(III), As(V)) to 10 -g/L (DMA, MMA), with recovery rates between 97% and 115%. For sediment matrices, the calibration range was set between 30 and 150 microg/L using a Hamilton PRP X100 column. The mobile phase employed was (NH4)2CO3 in a gradient concentration ranging from 20 mM to 50 mM. The resulting limits of quantification were within 0.05 mg/kg-0.07 mg/kg, while recovery rates ranged from 67% to 91%.
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