Scholarly record
NEUROVASCULAR UNIT (NVU) IN HEALTH AND DISEASE. ANALYSIS IN THE CONTEXT OF PATHOLOGICAL- BLOOD-BRAIN-BARRIER (BBB) ON CHIP MODELSESTABLISHMENT FOR NEW DRUGS DEVELOPMENT AND SCREENING
Abstract
Organs-on-chip allow for establishment of physiologically relevant models of human tissues and organs, and specifically of blood-brain barrier (BBB) and neurovascular unit (NVU) in their normal or pathological state. BBB/NVU integrity and functions are compromised in course of different diseases or injuries, like traumatic brain injury, stroke, multiple sclerosis, arteriovenous malformations, vascular dementia, Alzheimer?s disease, atherosclerosis, brain cancer, inflammation, genetic disorders, and many more. In this review we discuss main BBB/NVU properties, and latest achievements in healthy and pathological human BBB microfluidic models development. The growing evidence indicates that for human in vitro BBB models (and especially when recreating pathology) it is beneficial to use human induced pluripotent stem cells (iPSC). Besides the right choice of cells, it is obvious that static in vitro models are far from physiology, that?s why microfluidic human iPSC-based BBB models are of particular interest. Such models are potentially useful for development and testing of new neuropharmaceuticals, and development of strategies to prevent the breakdown of BBB/NVU in the course of certain disease.
Publication Impact Profile
Publication details
References13
Netto JP, Iliff J, Stanimirovic D, Krohn KA, Hamilton B, Varallyay C, и др. Neurovascular Unit: Basic and Clinical Imaging with Emphasis on Advantages of Ferumoxytol. Neurosurgery. 1 июнь 2018 г.;82(6):770–80.
Muoio V, Persson PB, Sendeski MM. The neurovascular unit - concept review. Acta Physiol. апрель 2014 г.;210(4):790–8.
Shabir O, Berwick J, Francis SE. Neurovascular dysfunction in vascular dementia, Alzheimer’s and atherosclerosis. BMC Neurosci. декабрь 2018 г.;19(1):62.
Bosworth AM, Faley SL, Bellan LM, Lippmann ES. Modeling Neurovascular Disorders and Therapeutic Outcomes with Human-Induced Pluripotent Stem Cells. Front Bioeng Biotechnol. 30 январь 2018 г.;5:87.
Noumbissi ME, Galasso B, Stins MF. Brain vascular heterogeneity: implications for disease pathogenesis and design of in vitro blood–brain barrier models. Fluids Barriers CNS. декабрь 2018 г.;15(1):12.
Abdullahi W, Tripathi D, Ronaldson PT. Blood-brain barrier dysfunction in ischemic stroke: targeting tight junctions and transporters for vascular protection. Am J Physiol Cell Physiol. 1 сентябрь 2018 г.;315(3):C343–56.
Sweeney MD, Sagare AP, Zlokovic BV. Blood-brain barrier breakdown in Alzheimer disease and other neurodegenerative disorders. Nat Rev Neurol. март 2018 г.;14(3):133–50.
Fiorentino M, Sapone A, Senger S, Camhi SS, Kadzielski SM, Buie TM, и др. Blood–brain barrier and intestinal epithelial barrier alterations in autism spectrum disorders. Mol Autism [Интернет]. 29 ноябрь 2016 г. [цитируется по 24 май 2019 г.];7. Доступно на: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129651/
Hollmann EK, Bailey AK, Potharazu AV, Neely MD, Bowman AB, Lippmann ES. Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cells. Fluids Barriers CNS. декабрь 2017 г.;14(1):9.
Logan S, Arzua T, Canfield SG, Seminary ER, Sison SL, Ebert AD, и др. Studying Human Neurological Disorders Using Induced Pluripotent Stem Cells: From 2D Monolayer to 3D Organoid and Blood Brain Barrier Models. Compr Physiol. 14 март 2019 г.;9(2):565–611.
Wang YI, Abaci HE, Shuler ML. Microfluidic blood-brain barrier model provides in vivo-like barrier properties for drug permeability screening: Microfluidic BBB Model Mimics In Vivo Properties. Biotechnol Bioeng. январь 2017 г.;114(1):184–94.
Faley SL, Neal EH, Wang JX, Bosworth AM, Weber CM, Balotin KM, и др. iPSC-Derived Brain Endothelium Exhibits Stable, Long-Term Barrier Function in Perfused Hydrogel Scaffolds. Stem Cell Rep. март 2019 г.;12(3):474–87.
The Original Human-on-a-Chip Company [Интернет]. Hesperos Inc. [цитируется по 19 май 2019 г.]. Доступно на: https://hesperosinc.com/
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.
