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INFLUENCE OF SOIL APPLICATION OF BIOCHAR, A FERTILIZER, A BIOPREPARATION AND A PESTICIDE ON THE FUNCTIONAL CHARACTERISTICS OF SOIL MICROBIAL COMMUNITY
Abstract
In the present study, we estimated the influence of chicken manure derived biochar prepared at 400?C during 2 h being introduced into soil at the dose of 30 t ha-1 on the functional diversity of soil microbes. The experiment was carried out in laboratory conditions in 10L soil containers. To estimate that, substrate utilization ability of the communities were analyzed using Biolog Ecoplates® method. Biochar introduction was analyzed solo as well as with the co-influence of the following factors: granulation, addition of 1 * 106 CFU g-1 of consortium of microorganisms (Azospirillum zeae, Bacillus megaterium, Pseudomonas fluorescens, Pseudomonas putida); addition of 0.35 l*ha-1; of a pesticide containing propiconazole 300 g*l-1 and tebuconazole 200 g*l-1; addition of 40 g*m-2 of a composite fertilizer containing 10% of nitrogen, and 26% of P2O5 and K2? each. All parameters were estimated on the 1st, 7th, 14th and 28th days of the experiment. Soil with no treatment was used as a control. It was revealed, that ?AWCD values for all experimental samples were 1.1 ? 2.1 times higher than those of control at all the four time points. The highest ?AWCD were observed on the 1st day of the experiment for microbe amended soil as well as fertilizer and pesticide amended soil (1.7 and 2.1, correspondingly). The application of the fertilizer, the pesticide and the microbes led to the increase of fluctuation amplitudes of ?AWCD as compared with control while the co-application of biochar smoothed those amplitudes. The application of powder and granular biochars demonstrated equal trends of influence on the functional microbial diversity.
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