Scholarly record
CONDITIONS OF AEROGENIC EXPOSITION TO BENZOL AND GENETIC STATUS AS FACTORS OF FORMATION OF IMMUNE PROFILE FEATURES IN MEN WITH VEGETATIVE REGULATION IMPAIRMENTS
Abstract
Introduction Violations of autonomic nervous regulation are a multifactorial pathology, while environmental features are an important risk factor for this pathology. Excessive exposure to neurotropic technogenic chemical factors, including benzene, to the biological environment of the human body induces malfunctions of adaptive-compensatory mechanisms and forms an increased risk of developing disorders of autonomic regulation associated with the immune system, which are realized against the background of polymorphism of candidate genes. Purpose of the work: analysis of the characteristics of the immunological and genetic status of men with disorders of autonomic nervous regulation under conditions of aerogenic exposure to benzene. Materials and methods 122 men living in conditions of excessive hapten load with benzene were examined. The observation group consisted of 69 men diagnosed with autonomic dysfunction syndrome. The comparison group consisted of 53 men without clinical manifestations of the pathology of the nervous system. Determination of CD127-, CD3+CD95+-lymphocytes, TNFR, p53 was carried out by flow cytometry. The determination of specific IgG to benzene was carried out using an allergosorbent test with an enzyme label. The identification of single nucleotide polymorphic variants (SNPs) of the immune response gene FOXP3 (rs3761547) and the gene for the antioxidant enzyme superoxide dismutase SOD2 (rs2758330) was carried out using modern biomedical technologies by real-time PCR. Results and discussion The group of men with pathology of the autonomic nervous system - autonomic dysfunction syndrome, living under conditions of aerogenic exposure to benzene, is characterized by an increased level of blood contamination with benzene, 1.5 times higher than that in the comparison group (p <0,05). The immune profile is characterized by an increase in the level of specific sensitization (IgG to benzene), activation of apoptosis (TNFR, p53) and phagocytosis relative to the comparison group. An increased (p <0.05) level of serum IgA production was established in relation to the comparison group. The content of CD127-lymphocytes significantly (p <0.05) exceeds the reference level against the background of a significantly reduced (p <0.05) level of CD3+CD95+-lymphocytes, regardless of the presence of pathology of the autonomic system in men with excessive haptenic load with benzene. The revealed features of the immune status of men with autonomic regulation disorders were significantly associated (OR>1; p<0,05) with the variant allele of the FOXP3 immune regulation gene (rs3761547), as well as the wild-type allele of the SOD2 superoxide dismutase gene (rs2758330) and the corresponding homozygous genotypes. Conclusion The established features of immune regulation (hyperproduction of IgG to benzene, imbalance of markers of apoptosis CD127-, CD3+CD95+, p53, TNFR) against the background of altered polymorphism of candidate genes (FOXP3, SOD2) form a complex of genetic and immunological markers for the development of disorders of autonomic regulation in men living under conditions of aerogenic exposure to benzene.
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