Scholarly record
HRP IMMOBILIZATION IN BIOCOMPATIBLE SOL-GEL MATRICES
Abstract
Horseradish peroxidase (HRP) is an enzyme belonging to the oxidoreductase class with applications in biotechnology and the food industry, environmental depollution and clinical diagnosis. HRP is an effective biocatalyst in the oxidation of organic compounds. Many biotechnology fields, such as environmental applications and the pharmaceutical industry, require stable peroxidase-based biocatalytic systems. The goal of our work was to develop efficient immobilized biocatalysts containing HRP. HRP immobilization was performed in biocompatible silica matrices obtained by the sol-gel method. Two precursors were used - tetramethoxysilane and tetraethoxysilan. Ethanol has been used as a classical co-solvent in sol-gel methods. For better protection of enzyme molecules, ethylene glycol has also been tested in soil synthesis as an alternative to ethanol. The immobilization of HRP in alkoxysilane matrices was done with good immobilization yields. The gels containing the immobilized enzymes had a good stability over time, being effective systems for the controlled release of the enzyme.
Publication Impact Profile
- Captures
- Mendeley - Readers: 1
Publication details
References14
Mohamed S.A., Elsayed A.M., Salah H.A., Barakat A.Z., Bassuiny R.I., Abdel-Mageed H.M., Abdel-Aty A.M., Development of chia gum/alginate-polymer support for horseradish peroxidase immobilization and its application in phenolic removal, Scientific Reports, 2024, 14, pp. 1362 DOI: 10.1038/s41598-024-51566-x
Lim G.T., Jo B.H., Exploiting Silica-Binding and Silica-Forming Proteins as Versatile Tools for One-Step Enzyme Immobilization on Siliceous Materials, International Journal of Molecular Sciences, Switzerland, 2025, pp. 1304, ISSN 1422-0067, DOI: 10.3390/ijms26031304
del-Bosque D., Vila-Crespo J., Ruip�rez V., Fernandez-Fernandez E., Rodriguez- Nogales J.M., Silica-Calcium-Alginate Hydrogels for the Co-Immobilization of Glucose Oxidase and Catalase to Reduce the Glucose in Grape Must, Gels, Switzerland, 2023, pp. 320, ISSN 2310-2861, DOI: 10.3390/gels9040320
Pota G., Sapienza Salerno A., Costantini A., Silvestri B., Passaro J., Califano V., Co-immobilization of cellulase and �-glucosidase into mesoporous silica nanoparticles for the hydrolysis of cellulose extracted from Eriobotrya japonica leaves, Langmuir, USA, 2022, pp. 5481�5493, ISSN 0743-7463, DOI: 10.1021/acs.langmuir.2c00053
Antunes A., Oro C.E.D., Denti A.F., da Silva L.M., Ficanha A.M.M., Mulinari J., Venquiaruto L.D., Zeni J., Mignoni, M.L., Dallago, R. M. (2024). In situ CALB immobilization in xerogel and sonogel employing TMOS as silica precursor and polyethylene glycol as additive, Processes, 12(11), 2411. https://doi.org /DOI: 10.3390/pr12112411
Weiser D., Nagy F., Ban�czi G., Olah M., Farkas A., Szilagyi A., Laszl� K., Gell�rt �., Marosi G., Kem�ny S., Poppe L., Immobilization engineering � How to design advanced sol�gel systems for biocatalysis?, Green Chem., 2017, 19(16), pp. 3927-3937, DOI: 10.1039/C7GC00896A
Reetz MT, Tielmann P, Wiesenh�fer W, K�nen W, Zonta A., Second generation sol�gel encapsulated lipases: robust heterogeneus biocatalysts, Adv. Synt. Catal., 2003, 345, pp. 717�728. DOI: 10.1002/adsc.200303016
Dragomirescu M., Vintila T., Vlase T., Mihali C.V., Preda G., Microbial cellulases immobilized in biopolymer/silica matrices used as enzyme release systems, Acta Biochimica Polonica, 2021, 68(4), pp 775-783, DOI: 10.18388/abp.2020_5694
Preda G., Peter F., Dragomirescu M., Biocatalizatori enzimatici: obtinere, caracterizare, aplicatii, Editura Mirton, Timisoara, Romania, 2003
Horseradish Peroxidase Assay Protocol
Fukuoka M., Makishima A., Effects of the Type of Alcoholic Solvent on the Formation of SiO2 Wet Gels in Sol-Gel Process, Journal of the Ceramic Society of Japan, 2003, 111(1290), pp 83-86, Released on J-STAGE March 28, 2003, Online ISSN 1882-1022, Print ISSN 0914-5400, https://doi.org/10.2109/ jcersj. 111.83, https:// www.jstage.jst.go.jp/article/jcersj/111/1290/111_1290_83/_article/-char/en DOI: 10.2109/jcersj.111.83
Fuentes K.M., Coria-Oriundo L.L., Wirth S., Bilmes S.A., Functionalized hierarchical wrinkled-silica spheres for laccases immobilization, J Porous Mater, 2021, 28, pp. 261�269 DOI: 10.1007/s10934-020-00988-9
Singh R., Jha A.B., Sharma P., Entrapment of enzyme in the presence of proline: effective approach to enhance activity and stability of horseradish peroxidase, 3 BIOTECH, 2020, WOS:000525106700006. DOI: 10.1007/s13205-020-2140-7
V Janovic BS, Micic Vicovac ML, Vujcic ZM, Vujcic MT., Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes., Environ Sci Pollut Res Int.,2017, 24(4), pp. 3923-3933. DOI: 10.1007/s11356-016-8100-4
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.