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THE CHITOSAN STABILIZATION OF THE PROTEASES AND CONSTITUENT OF THE H. SALINARUM CELL LYSATE DURING DRYING AND LONG-TERM STORAGE
Abstract
The study of the properties and immobilization of various therapeutic agents on natural carriers is an actual task of medical biotechnology and bioorganic chemistry, since this allows creation of stabilized, biologically active drugs with controlled release, as well as a targeted site of action for use in various fields of medicine. Such therapeutic agents can be used as cleansing, antibacterial and (or) antioxidant treatments. The loss of biological activity of the materials, which contains various therapeutic agents during the preparation, drying, storage and use of drugs by patients under the influence of human body temperature, pH of the wound and various inhibitors, is one of the core problems of their production. In medical practice, the co-use of hydrolases, biocides, antioxidants, and more recently, immunostimulants and immunomodulators is indicated for the treatment of purulent-necrotic diseases. Enzymes are the most labile of these substances. Therefore, there is an acute problem of the mutual influence and preservation of the medicines' biological properties after synthesis. Immobilization of proteases on a chitosan carrier can reduce the inactivation possibility at the stages of preparation, storage, and use of the drug. As we have found, many therapeutic agents inactivate the proteases used in the work, especially at temperatures above 25 ?C. Immobilization in chitosan gel helps to preserve of biological activity (there is a decrease in the effective rate constant of inactivation), especially during thermal inactivation (37 ?C) and drying. Immobilization in chitosan gel, drying, and storage of various therapeutic agents and their mixtures act on the drug release kinetics in different ways.
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