Scholarly record

STUDY OF THE KINETIC OF BIOCATALYTIC REACTIONS USING MAGNETICALLY SEPARABLE OXIDOREDUCTASES

Olga Grebennikova, Aleksandrina Sulman, Alyona Sokolova, Boris Tikhonov, Valentina Matveeva

Abstract

Oxidoreductase enzymes play a key role in modern biotechnology due to their high specificity and ability to catalyze redox processes under mild conditions. They are used in environmental monitoring, biosensor development, and fine organic synthesis. However, their practical use in their native form is limited by their low stability, sensitivity to environmental changes, and the inability to re-extract from the reaction mixture. Immobilizing enzymes on various carriers can solve these problems. Magnetic nanoparticles are currently a promising carrier for enzymes, as magnetically separable biocatalysts allow for easy separation of the enzyme from the reaction products using an external magnetic field. In this work, we studied the kinetic parameters of oxidation reactions using glucose oxidase and peroxidase immobilized on Fe3O4 magnetic nanoparticles. The surface of the magnetic nanoparticles was pretreated with tetraethoxysilane, 3-minopropyltriethoxysilane, and glutaric dialdehyde. A glucose oxidase-based biocatalyst was studied in the oxidation of glucose. Biocatalytic systems using peroxidase were evaluated in the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)ammonium with hydrogen peroxide. Study of kinetics of such systems is critical for understanding the efficiency of the biocatalyst and predicting its behavior in real technological processes. In this work, the Michaelis constant and the maximum reaction rate were determined for all the obtained biocatalyst samples.

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