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SYNTHESIS OF MAGNETICALLY SEPARATED BIOCATALYTIC SYSTEMS
Abstract
The use of magnetic nanoparticles in biocatalysis, due to their unique properties, such as controlled particle size, large surface area, and ease of separating them and the reaction mixture by applying an external magnetic field, makes it possible to reuse enzymes immobilized on magnetic nanoparticles for catalytic processes. In this work, horseradish root peroxidase was immobilized on Fe3O4 magnetic nanoparticles. The carrier surface was modified and activated before enzyme immobilization using 3- aminopropyltriethoxysilane and glutaraldehyde. Testing of biocatalytic systems was carried out in the oxidation reaction of 2,2'-azino-bis-(3-ethylbenzthiozolin-6-sulfonic acid) diammonium salt with hydrogen peroxide. The immobilized enzyme showed high efficiency and stability compared to the native enzyme. Also, in the work, the joint immobilization of peroxidase and glucose oxidase on magnetically attached carriers was studied. Enzymes were immobilized on Fe3O4 magnetic nanoparticles and SiO2. Optimal conditions (temperature, pH) were selected for all biocatalytic systems.
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