Scholarly record
EFFECT OF DIETARY FATTY ACIDS ON PHENOTYPES OF IMMUNE CELLS
Abstract
Abnormal numbers of specific types of leukocytes may indicate immuno-suppression or immunocompetence in response to an immunomodulator. The objective of this study is to investigate the effect of feeding broilers on diet containing flaxseed on splenocyte Tand B- cells. Upon hatching, all chicks were given the same basal diet for 13 d. Dietary supplementation of flaxseed started at 14 d of ages until the end of the cycle at 35 d of age. At slaughter, samples of spleen were collected. Spleen cells were harvested in cell suspensions. Immune cells were then enumerated using the flow haemocytometer in the Physiology Laboratory. The overall differences between the dietary treatments were analysed using one-way analysis of variance (ANOVA) and the general linear model procedure of Minitab. Statistically, there was no significant effect of flaxseed on the percentage positive or mean fluorescence intensity of the leukocyte subsets under investigation. However, there was a trend towards an increase in the proportion of Bcells in the spleen after feeding 15 % of flaxseed, which approached significance (P=0.058). There was also a trend towards a decrease in the mean fluorescence intensity of CD8+ subsets in the spleen, which was close to statistical significance (P=0.054).This trend is particularly interesting, given the fact that the bursii in these chickens were significantly observed to be smaller and thinner. It suggests that flaxseed may either prevent the homing of B-lymphocytes to the bursa, or encourage the release of Blymphocytes from the bursa into the circulation and then in the spleen.
Publication Impact Profile
Publication details
References18
Uchiyama, R., et al., Counting absolute number of lymphocytes in quail whole blood by flow cytometry. Journal of Veterinary Medical Science, 2005. 67(4): p. 441-444.
Davis, A.K., Effect of handling time and repeated sampling on avian white blood cell counts. Journal of Field Ornithology, 2005. 76(4): p. 334-338.
Michael Ferris, M. and W.J. Bacha, A New Method for the Identification and Enumeration of Chicken Heterophils and Eosinophils. Avian diseases, 1984. 28(1): p. 179-182.
Fredrickson, T.N. and H.L. Chute, Further studies of chicken blood tests and thier application, in Department of animal pathology. 1957, University of Maine: Orono. p. 9.
Fredrickson, T., N. , H.L. Chute, and D.C. O'meare, Preliminary Investigations on the hematology of broiler flocks, in 28th annual meeting of the Northeastern conference of laboratory workers in Pullorum disease control. 1956, University of Delaware: Newark, Delaware.
Blain, D., A study of the white blood cells of the normal fowl by the spravital technique. 1928, Henry Strong Denison Medical Foundation: The department of anatomy, Vanderbilt university, School of medicine, Nashville, Tennessee.
Gross, W.B., Differential and total avian blood cell counts by the hemacytometer method. Avian/Exotic Practice, 1984. 1(2): p. 31-36.
Hauska, H., et al., Comparison of different staining methods for avian blood cells. [German]. Tierarztliche Praxis, 1999. 1999. 27(4): p. 280-287.
. Pintea, V. and E. Gersak, Avian blood cells from the 7th day of incubation to the 6th day after hatching. [Romanian]. Lucrari Stiintifice Institutul Agronomic Timisoara, Medicina Veterinara, 1984. 19: p. 38-43.
Brennan, C.P., et al., Melatonin and the enhancement of immune responses in immature male chickens. Poultry Science, 2002. 81(3): p. 371-375.
Eeva, T., et al., Pollution related effects on immune function and stress in a free-living population of pied flycatcher Ficedula hypoleuca. JOURNAL OF AVIAN BIOLOGY, 2005. 36: p. 405-412.
Swamy, H.V.L.N., et al., Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on growth and immunological parameters of broiler chickens. Poultry Science, 2004. 83(4): p. 533-543.
Mashaly, M.M., et al., Effect of heat stress on production parameters and immune responses of commercial laying hens. Poultry Science, 2004. 83(6): p. 889-894.
Wang, Y.W., C.J. Field, and J.S. Sim, Dietary polyunsaturated fatty acids alter lymphocyte subset proportion and proliferation, serum immunoglobulin G concentration, and immune tissue development in chicks. Poult Sci, 2000. 79(12): p. 1741-1748.
Oth, D., et al., Modulation of CD4 expression on lymphoma cells transplanted to mice fed (n - 3) polyunsaturated fatty acids. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1990. 1027(1): p. 47-52.
Yang, X. J. and Guo, Y., Modulation of intestinal mucosal immunity by dietary polyunsaturated fatty acids in chickens. Food and Agricultural Immunology, 2006. 17: p. 129-137.
Selvaraj, R. K. and Cherian, G., Changes in delayed type hypersensitivity, egg antibody content and immune cell fatty acid composition of layer birds fed conjugated linoleic acid, n-6 or n-3 fatty acids. Canadian Journal of Animal Science, 2004. 84: p. 221-228.
Yang, X., Guo, Y., He, X., Yuan, J., Yang, Y. and Wang, Z., Growth performance and immune responses in chickens after challenge with lipopolysaccharide and modulation by dietary different oils. Animal, 2008. 2: p. 216-223.
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.
