Scholarly record
EFFECT OF EXTRACTING SOLVENT VOLUME ON RECOVERY RATE OF TWO VETERINARY ANTIBIOTICS ADDED TO SWINE MANURE
Abstract
Land application of swine manure can increase antibiotic concentra tions in agricultural soils. It is thus important to develop labor atory methods for antibiotics in manures and soils. The objective of this study was to examine the effect of volume of Na 2EDTA– McIlvaine’s buffer (c itric acid and Na 2HPO4) solution on recovery of oxytetracycline (OTC) and chlortetracycline (CTC) added to fresh swine manur e. Antibiotics were mixed with manure at a single rate of 20 mg /L. Six treatments (8, 16, 24, 32, 40 or 48 ml) of extracting agents were used as 0.2 M Na 2EDTA + 0.2 M citric acid + 0.4 M Na2HPO4 solution to extract CTC and OTC fr om swine manure compost. Antibiotics concentration in manure extracts was analyzed by high performance liquid chromatography with ultraviolet detection. Co mpared with control (8 ml), the highest rate (48 ml) of Na 2EDTA-McIlvaine’s buffer solution increased the extraction recovery of CTC and CTO by a factor of 1.8 and 1.4, respectively. There was lesser recovery of CTC than OTC. At the levels of 8 and 48 ml of extracting solution mean recovery rates were 59.7±14.2 and 81.8±16.0 % for OTC, and 36.1±12.1 and 65.7±0.1% for CTC, respectively. The concentration of CTC and OTC in fresh swine manure compost must account for a recovery factor that depended on volume of extracting solution.
Publication Impact Profile
Publication details
References13
Hamscher G., Sczesny S., Höper H. & Nau H. Determination of persistent tetracycline residues in soil fertilized with liquid manur e by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Analytical Chemistry, vol. 74, pp 1509–1518, 2002.
Blackwell P.A., Holten Lützhøft H.-C., Ma H.-P., Ha lling-Sørensen B., Boxall A.B.A. & Kay P. Ultrasonic extraction of ve terinary antibiotics fr om soils and swine slurry with SPE clean-up and LC-UV and fluor escence detection. Talanta, 64, pp 1058– 1064, 2004.
Wang Q. & Yates S.R. Laboratory study of oxytetracycline degradation kinetics in animal manure and soil. Journal of Agricultural and Food Chemistry, vol. 56, pp 1683 – 1688, 2008.
Parent E. Détermina tion quantitative d’antibiotiques (chlortétracycline, oxytétracycline, tylosine). Mémoire de maîtrise, Département des sols et génie agroalimentaire, Université Laval. Québec, Canada, 2009.
Ramos L., Kristenson E.M. & Brinkman U.A.T. Journal of Chromatography, vol. 975A, pp 3–29, 2002. Ecology and Environmental Protection
Lunestad B.T. & Goksoyr J. Reduction in the antibacterial effect of oxytetracycline in sea water by complex formation with ma gnesium and calcium. Diseases of Aquatic Organisms, vol. 9, pp 67–72, 1990.
Samuelsen O.B., Torsvik V. & Ervik A. Long-range changes in oxytetracyline concentration and bacterial resistance toward s oxytetracycline in a fish farm sediment after medication: The Science of the Total Environment, vol. 114, pp 25–36, 1992.
Tolls J. Sorption of Veterinary Pharmaceuticals in Soils: Environmental Science and Technology, vol. 35, pp 3397–3406, 2001.
Jin L., Amaya-Mazo X., Apel M.E., Sanki sa S.S., Johnson E., Zbyszynska M.A. & Han A. Ca 2+ and Mg 2+ bind tetracycline with distin ct stoichiometries and linked deprotonation. Biophysical Chemistry, vol. 128, pp 185-196, 2007.
Kulshrestha P., Giese R.F. & Aga D.S. Investigating the molecu lar interactions of oxytetracycline in clay and organic matter: insights on factors affecting its mobility in soil. Environmental Science and Technology, vol. 38, pp 4097–4105, 2004.
Mackay A.A. & Canterbury B. Oxytetracycline sorption to organic matter by metal-bridging. Journal of Environmental Quality, vol. 34, pp 1964–1971, 2005.
Pils J.R.V. & Laird D.A. Sorption of te tracycline and chlortetracycline on K- and Ca-saturated soil clays, humic substances, and clay–humic complexes. Environmental Science and Technology, vol. 41, pp 1928–1933, 2007.
Vilarem G., Dumont V. & Gaset A. Ultr asonic assisted extraction. Oléagineux, Corps Gras, Lipides, vol. 4, pp 42-45, 1997.
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.