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RESEARCH ON THE ANTIMICROBIAL EFFECT OF ALLIUM SATIVUM EXTRACT ON SOME STRAINS OF SALMONELLA SPP ISOLATED FROM DOGS
Abstract
In recent years, the development of resistance to various antibiotics frequently used in veterinary practice has been observed. The cause seems to be the excessive use of antibiotics without doing an antibiogram. The aim of this study was to investigate the antimicrobial effect of different garlic extracts on Salmonella strains isolated from dogs. The research was carried out on a number of 19 strains of Salmonella spp. isolated from fecal samples from dogs. Salmonella spp is a bacterium with zoonotic risk. Different concentrations of alcoholic extract of Allium Sativum (100%, 50%, 25%), aqueous extract of garlic, and oil extract of garlic were tested on the 19 strains of Salmonella spp., isolated from fecal samples from dogs. Antimicrobial effect testing of Allium Sativum extract was performed on Mueller Hinton agar. In the case of the alcoholic extract of Allium Sativum 100% and in the case of garlic oil (25 mg Allicin), the highest value of the diameter of the inhibition zone (30 mm) was recorded. The lowest value of the zone of inhibition was recorded, in the case of the 25% alcoholic extract of garlic, approximately 11 mm. The aqueous extract of Allium Sativum had the diameter of the zone of inhibition variable from one strain to another, with values between 22 mm and 29 mm. We recommended the use of the alcoholic extract of Allium Sativum 100 % and the same amount of garlic oil with a concentration of 25 mg Allicin, in case of infection with Salmonella spp. with multiple resistance to antibiotics.
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References13
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