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HYDROCARBON OXIDIZING BACTERIAL STRAIN - ISOLATE FROM EISENIA FETIDA ANDREI (BOUCHE, 1972) INTESTINE
Abstract
Due to their huge volumes, toxicity and destruction resistance petroleum and petroleum products are the most important polluters. The source of oil pollution is not only oil extraction processes, but also oil transportation, refining and use. Ecologically safe and effective way to clean the environment off petroleum hydrocarbons is bioremediation. The effectiveness of oil-oxidizing biological products is ultimately determined by the activity of contained hydrocarbon oxidizing bacteria strains. The search, isolation and research of these strains are continual. The source of extraction of oil-oxidizing microorganisms is natural and anthropogenically modified ecosystems polluted by petroleum hydrocarbons. The objective of this work is the assessment of possibility of isolating degrading microorganisms from soil oligochaetes. As follows from microbiological analysis of red Californian hybrid Eisenia fetida andrei (Bouche, 1972) intestine, the bacterial strain Dch1 was isolated on selective medium for oil-oxidizing microorganisms. Based on morphologically-cultural and physiologically-biochemical characteristics, the isolate was assigned to Rhodococcus sp. The isolated strain grows on media containing hexane, octane, undecane, dodecane, hexadecane, benzene, naphthalene, petroleum, gasoline, diesel, jet fuel and fuel oil as the only carbon and energy source. Isolate Dch1 has lipolytic, amylolytic and urease activities. The ability of the studied strain to generate electric current in biofuel cells is shown.
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