Scholarly record
REGENERATION POTENTIAL OF TOMATO CALLUS CULTURE
Abstract
The purpose of the research was to study the regenerating potential of tomato callus culture depending on the hormonal composition of the medium, genotype, and age of the explant. Introduction in vitro culture was performed according to the standard method. As a basis, the mediums were served -Murashige and Skoog (MS) and Linsmaier and Skoog (LS). Growth hormones were added in various combinations and concentrations. As initial material, samples of local and foreign selection tomato varieties. As explants, cotyledons, hypocotyl, flower buds, parts of the leaf and stem of a vegetative plant were used. The regeneration efficiency was determined after 5 weeks from the start of cultivation as the ratio of the number of shoots (more than 8 mm in length) to the total number of explants. We have evaluated and optimized conditions in a vitro culture that affected the regeneration of shoots from various organs and tissues of tomato. Studies had shown that morphogenetic potential depended on explant, cultivation conditions, and genotype. The greatest regeneration potential was observed in the Big Star F1 hybrid with the cultivation of 6-daily hypocotyl segments 589.2±14.3%. The optimal nutrient medium for intensive callusogenesis and regeneration for the variety samples BigStar F1, Etude and Zvartnots was MS medium, supplemented with BAP - 3.0 mg/l and IAA - 0.5 mg/l.
Publication Impact Profile
Publication details
References28
Aazami M.A. Effect of some growth regulators on “in vitro” culture of two Vitis vinifera L. cultivars /Rom. Biotechnol. Lett., vol. 15, pp 5229-5232, 2010
Abiri, R.; Valdiani, A.; Maziah, M.; Shaharuddin, N.A.; Sahebi, M.; Yusof, Z.N.B.; Atabaki, N.; Talei, D. A Critical Review of the Concept of Transgenic Plants. Insights into Pharmaceutical Biotechnology and Molecular Farming. Curr. Issues/ Mol. Biol. 2016, vol.18, pp 21–42.
Alexandrova K.S., P.D. Denchev and B. Y. Conger, “Micro Propagation of Switchgrass by Node Culture,” Crop Science, Vol. 36, No. 6, 1996, pp. 1709-1711.
Al-Juboory K.H., R.M. Skirvin, and D.J. Williams “Callus induction and adventitious shoot regeneration of gardenia (Gardenia jasminoides Ellis) leaf explants,” Scientia Horticulturae, vol. 72, no. 3-4, pp. 171–178, 1998.
Altman, A. 1999. The plant and agricultural biotechnology revolution: where do we go from here? Electronic Journal of Biotechnology 2(2) August 15. http://ejb.ucv.cl/content/vol2/issue2/full/1
Anwar N, Kikuchi A, Watanabe KN (2010) Assessment of somaclonal variation for salinity tolerance in sweet potato regenerated plants. African Journal of Biotechnology 9: pp 7256-7265.
Behbahani M., M. Shanehsazzadeh, and M. J. Hessami, “Optimization of callus and cell suspension cultures of Barringtonia racemosa (Lecythidaceae family) for lycopene production,” Scientia Agricola, vol. 68, no. 1, pp. 69–76, 2011.
Binte Mostafiz, S.; Wagiran, A. Efficient callus induction and regeneration in selected Indica rice. Agronomy 2018, vol. 8, pp 77.
Binzel ML, Sankhla N, Joshi S, Sankhla D (1996). Induction of direct somatic embryogenesis and plant regeneration in pepper (Capsicum annuum L.). Plant Cell Rep 15: pp.536–540.
Bohidar S., M. Thirunavukkarasu and T. V. Rao, “Effect of Plant Growth Regulators on in Vitro Micro Propagation of ‘Garden Rue’ (R. graveolens L.),” International Journal of Integrated Biology, Vol. 3, No. 1, 2008, pp. 36-43.
Butiuc-Keul A.L., Coste A., Halmagyi A., Deliu C., Farago M., Iliescu M. and Iuoras R., 2009. In vitro micropropagation of several grapevine cultivars from Romania. Acta Hortic., 812, pp.129-134.
Can E., Celiktas N. and Hatipoglu R., 2008. Effect of auxin type and concentrations in different media on the callus induction and shoot formation of crested wheatgrass (Agropyron cristatum (L.) Gaertn). Biotechnol. Biotechnol. Equip., 22, pp.782-786.
Gamborg OL, Phillips GC (1995) Media Preparation and Handling. In: Gamborg OL, Phillips GC (eds.), Plant Cell, Tissue and Organ Culture: Fundamental Methods. Springer, Germany, pp: 21-34.
Haliluev M.R., Kharchenko P.N., Dolgov S.V. Development of a regeneration system and the study of the transformational potential of an industrial tomato // Reports of the Russian Academy of Agricultural Sciences. 2010. No. 3. P. 22-26.
Hasbullah N.A., Saleh A. and Taha R.M., 2011. Establishment of somatic embryogenesis from Gerbera jamesonii Bolus ex. Hook F. through suspension culture. Afr. J. Biotechnol., 10, 13762- 13768.
Ives C. and Bedford B. (eds.) Agricultural biotechnology in international development Biotechnology in Agriculture 21. CAB International, Wallingford. pp. 368, 1998
Kalinin F.L., Sarnatskaya V.V., Polishchuk V.E. Methods of tissue cultivation in the physiology and biochemistry of plants. - Kiev, 1980. –485 p.
Linsmaier E. M., Skoog F. Organic growth factor requirement of tobacco tissue cultures. – Physiol. Plant., 1965, 18, N 1, p. 100-127.
Mok, M.C., Martin, R.C. and Mok, D.W.S. (2000). Cytokinin: biosynthesis, metabolism and perception. In Vitro Cellular Development of Biology Plant 36: 102- 107.
Mok, M.C., Mok, D.W.S., Turner, J.E. and Mujer, C.V. (1987). Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems. HortScience 22: 1194-1197.
Murashige T., Shabde M.N., Hasegawa P.M., Takatori F.H., Jones J.B. Propagation of asparagus through shoot apex culture. I. Nutrient medium for formation of plantlets. Journal of the American Society for Horticultural Science, 1972, pp.158 – 61.
Osman M.G., Khalafalla M.M. Promotion of in vitro shoot formation tip of tomato (Lycopersicon esculentum Mill, cv. Omdurman) by ethylene inhibitors //Internat. J. of Curr. Res. 2010b. V. 4. P. 082-086.
Rutkowska-Krause I. et al. Regeneration of flax (Linumus utatissimum L.) plants from antherculture and somatic tissue with increased resistance to Fusarium oxysporum// Plant Cell Rep. – 2003. – Vol.22, № 2. – pp 110–116.
Sayd S.S., H. A. A. Taie, and L. S. Taha, “Micropropagation, antioxidant activity, total phenolics and flavonoids content of Gardenia jasminoides Ellis as affected by growth regulators,” International Journal Academic Research, vol. 2, pp. 184–191, 2010.
Sugiyama M. Organogenesis in vitro. Current Opinion in Plant Biology 2: pp 61-64, 1999
Tehrim S., Mirza M.Y. and Sajid G.M., 2013. Comparative study of different growth regulators for efficient plant regeneration in grapes. Pakistan J. Agric. Res., 26, pp 275-289.
Vardja, R. and T. Vardja, 2001. The effect of cytokinin type and concentration and the number of subcultures on the multiplication rate of some decorative plants. Proc. Estonian Acad. Sci. Biol. Ecol., 50: pp 22-32.
Yerbolova L.S., Ryabushkina N.A., Oleichenko S.N., Kampitova G.A. and Galiakparov N.N. The effect of growth regulators on in vitro culture of some Vitis vinifera L. cultivars. World Appl. Sci. J., 23, pp76-80, 2013
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.
