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IMMOBILIZATION OF GLUCOSE OXIDASE ON MAGNETIC MESOPOROUS OXIDES
Abstract
The immobilization of glucose oxidase (GOx, enzyme of oxidoreductases class) was carried out on non-magnetic (SiO2, Al2O3) and magnetic mesoporous oxides (Fe3O4-SiO2, Fe3O4-Al2O3). Magnetic supports were synthesized by in-situ crystallization of magnetite nanoparticles in mesopores. The surface of the support was preliminarily modified with amino groups and the glutaraldehyde was used as a linker for covalent binding of the enzyme. The supports and biocatalysts were fully characterized using a combination of physicochemical methods (BET, TEM, DRIFT) and tested in D-glucose oxidation to D-gluconic acid. Magnetic support based biocatalysts demonstrate high catalytic activity in D-glucose oxidation at temperature of 30-50?C and pH 5-7.5 with the best activities of 95% and 91% for magnetic silica and alumina, respectively. A comparison of magnetic and non-magnetic alumina and silica shows a significant enhancement of the relative catalytic activity for magnetic supports.
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