Scholarly record
FUNGI CELLULASES FOR CRUDE FIBRE REDUCTION IN PLANT RAW MATERIALS
Abstract
Waste products of plant origin are valuable secondary raw materials in the process of agricultural wastes utilization into products with high added value. The application of secondary raw materials is complicated due to the high fibrecontent. The aim of this study was to identify strains of microorganisms capable to produce a wide range of exocellulolases in order to reduce the crude fibrein mild conditions without applying expensive commercial cellulases. The following strains were chosen for this experiment: Trichoderma viride and Trichoderma reesei according to the literature review. All the investigated strains have generally recognized as safe (GRAS) status. The effect of the Trichoderma viride culture fluid on the content of crude fibrein the process of heterogeneous submerged fermentation was studied as well as the effect of low lactose concentrations on the ability of microorganisms to produce enzymes. Determination of cellulase activity was carried by the classic method based on filter paper. Reducing sugars were detected according to the modified method of Miller. The content of crude fibrein the samples was determined by the method of Henneberg and Stohmann. Sunflower is widely used to produce vegetable oil and protein products in Russian Federation. Deproteinized sunflower meal with 24-29% crude protein and 39-42% crude fibrewas chosen as substrate.
Publication Impact Profile
Publication details
References14
Bailey M.J., Tähtiharju J. Efficient cellulase production by Trichoderma reesei in continuous cultivation on lactose medium with a computer-controlled feeding strategy // Applied microbiology and biotechnology. 2003. № 2–3 (62). P. 156–162.
ElBaz A.F. et. al. Optimization of cellulase production by Trichoderma viride using response surface methodology // Curr Biotechnol. 2016. (5). P. 1–7.
Jönsson L.J., Martín C. Pretreatment of lignocellulose: formation of inhibitory by-products and strategies for minimizing their effects // Bioresource technology. 2016. (199). P. 103–112.
Kaczmarek P. et. al. Chemical composition of rapeseed products and their use in pig nutrition // Polish Journal of Natural Sciences. 2016. № 4 (31). P. 545–562.
Kandari V. et. al. Cellulase and β-glucosidase production by Trichoderma viride and Aspergillus wentii in sub-merged fermentation utilizing pretreated lignocellulosic biomass // Journal of Microbiology and Biotechnology Research. 2017. № 5 (3). P. 63–78.
Kargopoulos A. et. al. Use of Sunflower Cake in the Diet of ISA Brown Pullets // Journal of Agricultural Science and Technology. 2017. (7). P. 129–134.
Lv D., Wang W., Wei D. Construction of two vectors for gene expression in Trichoderma reesei // Plasmid. 2012. № 1 (67). P. 67–71.
Madadi M., Tu Y., Abbas A. Pretreatment of Lignocelollusic Biomass Based on Improving Enzymatic Hydrolysis // International Journal of Applied Sciences and Biotechnology. 2017. № 1 (5). P. 1–11.
Matsumoto H. et. al. Cell dispersion culture for the effective growth of Humicola insolens and efficient enzyme production // Journal of bioscience and bioengineering. 2014. № 3 (117). P. 257–262.
Michelin M. et. al. Multi-step approach to add value to corncob: production of biomass-degrading enzymes, lignin and fermentable sugars // Bioresource technology. 2018. (247). P. 582–590.
Osmakova A., Kirpichnikov M., Popov V. Recent biotechnology developments and trends in the Russian Federation // New Biotechnology. 2017.
Palamae S. et. al. Cellulose and hemicellulose recovery from oil palm empty fruit bunch (EFB) fibers and production of sugars from the fibers // Carbohydrate polymers. 2017. (155). P. 491–497.
Srivastava N. et.al. Fungal Cellulases Production for Biodegradation of Agriculture Waste Springer, 2018. 75–89 P.
Tur A.V., Baurin D.V., Gordienko M.G., Shakir I.V. Panfilov.V.I. Optimisation of deproteinized plant raw material pretreatment conditions for obtaining fodder biomass 2017. 295–302 P.
View or Download full articleAccess options
SWS access login
Login as SWS Scientific CommitteeLogin as SWS Scientific PartnerLogin as SWS AuthorAuthors and approved SWS contributors will read and export their own linked papers after identity matching by SWS profile, email and SGEM GlobalID.
For librarian assistance: [email protected]
Purchase Instant Access
- Article can be downloaded after successful payment.
- Article may be used according to SWS library access terms.
- Article cannot be redistributed.
